The Small Animal Radiation Research Platform (SARRP; Xstrahl®; in collaboration with INFINITY lab, UGent) was used to irradiate ApoE−/− mice. Mice were anesthetized with isoflurane (5% induction and 2% maintenance) and subjected to a full body CT-scan (50 kV, 1.5 mA, 360 projections >360°, 1 mm aluminium filter) prior to irradiation. CT images were analyzed by using Muriplan software (Xstrahl®) to determine the coordinates of the isocenter for subsequent irradiation. Thoracic irradiation was performed in a ventro-dorsal direction, with 220-kV X-rays, operating at 13 mA and filtered with 0.15 mm of copper, operating with a dose rate of 3.4 Gy/min. The field size, created by the collimator of 10×10 mm, was encompassing both carotid arteries, the aortic arch and apical portion of the heart. The doses that were delivered to the heart region are 0 Gy (sham treatment; also referred to as ‘controls’), 0.1 and 10 Gy.
Muriplan software
Manufactured by Xstrahl
Sourced in United Kingdom
MuriPlan is a software application developed by Xstrahl to assist in the planning and execution of pre-clinical research involving irradiation of murine subjects. The software provides tools for treatment planning, dose calculation, and data management related to the irradiation process. MuriPlan is designed to streamline the workflow of researchers conducting radiation-based studies on small animal models.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using muriplan software
1
Murine Cardiovascular Radiation Protocol
2
Precision Radiotherapy Delivery in Preclinical Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anaesthetized with isoflurane and placed on an irradiator bed (SARRP, XStrahl Inc.). Treatment planning and radiation delivery were performed using MuriPlan software (XStrahl Inc.). CT images were acquired at 60 kV and 0.8 mA, with a voxel size of 0.275 mm, which delivers a dose of 1.2 cGy (21 (link)). Tissue segmentation was adjusted for each animal to define bone, tissue, and air. Field isocenters were applied to the segmented images to target radiotherapy delivery, and a collimator was used to customize the radiotherapy field. The SARRP delivers 225-kVp X-rays at 13-mA current (22 (link)), through a 0.15-mm Cu filter, at a dose rate of 3.1 Gy/min, and allows exact anatomic targeting with delivery of beams down to 0.5 mm. Mice received a 90° brain arc field (–45° to 45°), with a field size of 5×5 mm2. Animals were treated with 5 Gy per day for 4 days. Temozolomide (100 mg/kg) was administered by oral gavage 1 hour prior to irradiation. Treatment planning and delivery simulates the clinical situation (23 (link)). Mice were euthanized by cervical dislocation when they developed symptoms.
3
Subcutaneous Tumor Xenograft Irradiation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CAL27 cells (2 × 106) or SCC154 cells (7.5 × 106) were subcutaneously injected in each flank of 6–7-week-old female nu/nu Naval Medical Research Institute (NMRI) mice (Janvier Labs, France). The mice were either treated with a vehicle or RT (given in 5 fractions of 2 Gy, with a total dose of 10 Gy). RT was delivered via the Small Animal Radiation Research Platform (SARRP, X-strahl, Camberley, UK). Dose calculations were performed with MuriPlan software (X-strahl, Camberley, UK) after cone beam CT. The dose was delivered using 220 kV photons, 13 mA and a 10 × 10 mm collimator.
Tumor volumes (V = π/6xd_1xd_2xd_3) were determined with caliper measurements. The body weight and welfare of the mice were monitored daily during treatment and three times per week during follow-up. The experiment was performed according to the Ethical Committee for Animal Experimentation of KU Leuven (P163/2017; approval date 29 September 2017).
Tumor volumes (V = π/6xd_1xd_2xd_3) were determined with caliper measurements. The body weight and welfare of the mice were monitored daily during treatment and three times per week during follow-up. The experiment was performed according to the Ethical Committee for Animal Experimentation of KU Leuven (P163/2017; approval date 29 September 2017).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!