Mcf 7

Human breast cancer cell lines, MCF-7 and MDA-MB-453, were provided by the RIKEN BioResource Center (BRC) through the National Bio-Resource Project of the MEXT, Japan. Human breast cancer cell line, MDA-MB-231, and human mammary epithelial cell line, MCF10A, were purchased from the American Type Culture Collection (ATCC). MCF-7 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Nissui Pharmaceutical; Tokyo, Japan) supplemented with 10% inactivated fetal bovine serum (FBS; Biological Industries, Cromwell, CT, USA) in humidified atmosphere of 5% CO₂ at 37 °C. MDA-MB-453 and MDA-MB-231 cells were maintained in Leibovitz-L15 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% inactivated FBS in humidified atmosphere of 0% CO₂ at 37 °C. MCF10A cells were grown in mammary epithelial basal medium (MEBM; Lonza, Walkersville, MD, USA) supplemented with BPE, hydrocortisone, hEGF, insulin, gentamicin/amphotericin-B, and 100 ng/ml cholera toxin in humidified atmosphere of 5% CO₂ at 37 °C. 293FT cell line was purchased from Thermo Fisher Scientific (Waltham, MA, USA) and was maintained in DMEM supplemented with 10% inactivated FBS in humidified atmosphere of 5% CO₂ at 37 °C.

HeLa, MCF7, HEK-293T, MCF10A, and BT-549 cells were purchased from ATCC. HeLa, MCF7, and HEK-293T cells were maintained in DMEM (Nissui Pharmaceutical) supplemented with 8% FBS (Biowest) in an atmosphere with 5% CO₂. MCF10A cells were maintained in DMEM/F-12 (Sigma-Aldrich) supplemented with 5% FBS, 20 ng/mL human EGF (PeproTech), 10 μg/mL human insulin, and 0.5 μg/mL hydrocortisone (Tokyo Chemical Industry) in an atmosphere with 5% CO₂. BT-549 cells were maintained in RPMI1640 (Nissui pharmaceutical) supplemented with 8% FBS and 10 μg/mL human insulin (Sigma-Aldrich) in an atmosphere with 5% CO₂. Cell line identities were verified using the GenomeLab Human STR Primer set (Beckman Coulter). Cells were routinely stained by Hoechst 33342 and no mycoplasma contamination was suspected. Cells were used for experiments within 20 passages from obtaining.