Amino-5-phosphonopentanoic acid (D-APV), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), tetrodotoxin (TTX), BafilomycinA1, and SecinH3 were from Tocris Bioscience (Cookson, USA). Restriction enzymes were from New England Biolabs (Ipswich, USA). Cell culture media were from Invitrogen (Carlsbad, CA, USA). Soluble horseradish peroxidase (HRP) type VI, 3,3′-Diaminobenzidine (DAB) ( #P6782 and #D8001) and all other chemicals were from Sigma-Aldrich, St. Louis, MO. The following primary antibodies have been used: anti-GFP (#MAB3580; Millipore, Darmstadt, Germany); anti-synaptobrevin-2 (Syb2; #104 202; Synaptic Systems, Goettingen, Germany); anti-actin (#A4700) and anti-Arf6 (#A5230, Sigma); anti-Arf6 (#ARP54598_P050; Aviva, San Diego, CA); anti-Rab5(#108 011 Synaptic Systems); anti-Arf1 (#05–1427; EMD Millipore); anti-V5 (#60708; Invitrogen); anti-β-tubulin (#T8328; Sigma) anti-p38 (Mab5258, EMD Millipore); anti-Homer (#106 011; Synaptic Systems); anti-Vglut1 (#135 304; Synaptic Systems).
Anti homer
Manufactured by Synaptic Systems
Sourced in Germany
Anti-Homer is a laboratory instrument designed for the detection and quantification of the Homer protein. It utilizes advanced spectroscopic techniques to accurately measure the presence and concentration of this specific protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cell culture media, by Thermo Fisher Scientific (2 mentions)
Anti vglut1, by Synaptic Systems (2 mentions)
Anti gapdh, by Santa Cruz Biotechnology (2 mentions)
Anti v5, by Thermo Fisher Scientific (1 mentions)
Anti actin, by Merck Group (1 mentions)
Secinh3, by Bio-Techne (1 mentions)
Anti rab5, by Synaptic Systems (1 mentions)
Anti gfp, by Merck Group (1 mentions)
Anti β tubulin, by Merck Group (1 mentions)
Bafilomycin a1, by Bio-Techne (1 mentions)
Anti shank3, by Synaptic Systems (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Anti α synuclein, by BD (1 mentions)
Anti synaptophysin, by Synaptic Systems (1 mentions)
Anti glua1, by Abcam (1 mentions)
Anti flotillin 1, by BD (1 mentions)
Anti caveolin 1, by BD (1 mentions)
Anti synaptotagmin1, by Synaptic Systems (1 mentions)
Anti nmdar2b, by Abcam (1 mentions)
Anti psd 95, by Santa Cruz Biotechnology (1 mentions)
5 protocols using anti homer
1
Synaptic Protein Localization Assay
2
Immunostaining of Synaptic Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell culture media was from Invitrogen. Poly-L-lysine was from Sigma. The following primary antibodies were used: mouse monoclonal anti-Bassoon clone SAP7F407 (Abcam, UK), mouse monoclonal anti-Synapsin IIa (BD Biosciences, UK), rabbit polyclonal anti-Homer, rabbit polyclonal anti-Shank3, rabbit polyclonal anti-vGlut1 (Synaptic Systems, Germany). Secondary antibodies were from Jackson Immunoresearch (USA). APV, NBQX, TTX were from Tocris (UK).
3
Comprehensive Neuronal Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals, unless otherwise stated were purchased from Sigma-Aldrich (St. Louis, MO). The following primary antibodies were used: anti-PSD95 (Santa Cruz Biotechnology, Dallas, TX, catalog #SC-32290), anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, catalog #SC-25778) anti-flotillin1 (BD Biosciences, San Jose, CA, catalog #610821), anti-caveolin1 (BD Biosciences, San Jose, CA, catalog #610059), anti-α-synuclein (BD Biosciences, San Jose, CA, catalog #610787), anti-synaptophysin (Synaptic Systems, Goettingen, Germany, catalog #101011), anti-Homer (Synaptic Systems, Goettingen, Germany, catalog #160003), anti-synaptotagmin-1 (Synaptic Systems, Goettingen, Germany, catalog #105011) anti-NMDAR2a (Abcam plc, Cambridge, UK, catalog #ab133265), anti-NMDAR2b (Abcam plc, Cambridge, UK, catalog #ab28373), anti-NMDAR2b phospho S1480 (Abcam plc, Cambridge, UK, catalog #ab73014), anti-PKA (Santa Cruz Biotechnology, #sc-390548), and anti-GluA1 (Abcam plc, Cambridge, UK, catalog #ab32436). Syn peptides (amino acids 12–23 and 34–45) were obtained from Primmbiotech.
4
Characterizing Neuronal Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Standard immunocytochemistry and Western blot techniques were used to characterize cell type–specific markers and phenotypically relevant protein levels. The following antibodies were used for immunostaining according to the manufacturers’ instructions: anti-GFP (#1010; Aves Labs Inc), anti-PAX6 (PRB-278P; Covance), anti-Nestin (PRB-315C; Covance), anti-Map2 (#M2320; Sigma-Aldrich), and anti-Ctip2 (ab18465; Abcam). The following antibodies were used for Western blot assays: anti-Shank3 (sc30193; Santa Cruz), anti-Shank3 (sc 23547; Santa Cruz), anti-PSD95 (ab2723; Abcam), anti-Homer (#160011; Synaptic Systems), anti-NLGN1 (SAB1407153; Sigma-Aldrich), anti-PIKE (#07-675; Upstate), anti-Akt (#4691S; Cell Signaling), anti-phospho Akt (#4058; Cell Signaling), anti-mTOR (#4691; Cell Signaling), anti-phospho mTOR (#2971; Cell Signaling), anti-Jip2 (N135/37; NeuroMab), anti-NRP1 (SAB1411572; Sigma-Aldrich), anti-JNK1/3 (sc474; Santa Cruz), anti-phosphor JNK (#V793A; Promega), anti-DCX (#4604; Cell Signaling), anti-NeuN (#MAB377; EMD Millipore), anti-Tuj1 (#903401; BioLegend), anti-GAPDH (sc 47724; Santa Cruz), and anti-Actin (sc1616; Santa Cruz). Western blot quantification was performed using ImageJ.
5
Immunostaining and Confocal Imaging of Synaptic Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were stained as described53 (link). The following primary antibodies were used: anti-vesicular glutamate transporter-1 (VGLUT1, #135 304; Synaptic Systems, Gottingen, Germany), anti-Homer (#160011; Synaptic Systems), anti-vesicular GABA transporter (VGAT; #131003, Synaptic System), anti-Gephyrin (#147011; Synaptic System), anti-β-catenin (#PA5–19469; Thermo-Fisher Scientific), and guinea pig anti-Iba1 (#234004; Synaptic Systems). Fluorescently conjugated secondary antibodies were from Molecular Probes (Thermo-Fischer Scientific). Image acquisitions were performed using a confocal microscope (SP8, Leica Microsystems, Wetzlar, Germany) at ×63 (1.4 NA) magnification. Z-stacks were acquired every 300 nm, 10 fields/sample. 4,6-Diamidino-2-phenylindole staining was used to stain nuclei. The colocalization analysis was performed by evaluating the labeling of the VGLUT1/Homer1 or VGAT/Gephyrin synaptic protein couples. Co-localization puncta with areas of 0.1–2 mm2 were considered bona fide synaptic boutons. Synaptic boutons along neurites were manually counted on 30 μm stretches starting from the cell body.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!