Hearts were sectioned in the coronal plane to expose the four chambers, the aorta and the pulmonary trunk. The defected present in the LV apex represents the location of the catheter that was inserted to decompress the left ventricle during Langendorff type B perfusion (LV vent). Following each experiment, the heart was transferred to 10% neutral buffered formalin and paraffin embedded within 48 h. Using a microtome, 5 μm sections were prepared and stained with H&E, Masson’s trichrome (muscle: red; nucleus: blue; green/blue: collagen), and labeled with rabbit monoclonal recombinant anti-CD31 primary antibody (Abcam, Cambridge, UK) and fluorescent tagged with goat antirabbit IgG H&L (AF-647) preadsorbed secondary antibody (Abcam). The sections were also labeled with Isolectin Griffonia simplicifolia AF-488 conjugate (Thermo Fisher, Waltham, MA) which binds d-galactosyl residues of galactose α−1,3 galactose (Gal α−1,3 Gal). Nuclei were labeled with 4′,6-diamidina-2-phenylindole (DAPI).
Rabbit monoclonal recombinant anti cd31 primary antibody
Manufactured by Abcam
Sourced in United Kingdom
Rabbit monoclonal recombinant anti-CD31 primary antibody is a laboratory reagent used to detect the presence of the CD31 protein, also known as platelet endothelial cell adhesion molecule (PECAM-1), in biological samples. This antibody is produced recombinantly in rabbit cells.
Automatically generated - may contain errors
2 protocols using rabbit monoclonal recombinant anti cd31 primary antibody
1
Histological Analysis of Cardiac Tissue
2
Histological Analysis of Cardiac Tissue
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Hearts were sectioned in the coronal plane to expose the four chambers, the aorta and the pulmonary trunk. The defected present in the LV apex represents the location of the catheter that was inserted to decompress the left ventricle during Langendorff type B perfusion (LV vent). Following each experiment, the heart was transferred to 10% neutral buffered formalin and paraffin embedded within 48 h. Using a microtome, 5 μm sections were prepared and stained with H&E, Masson’s trichrome (muscle: red; nucleus: blue; green/blue: collagen), and labeled with rabbit monoclonal recombinant anti-CD31 primary antibody (Abcam, Cambridge, UK) and fluorescent tagged with goat antirabbit IgG H&L (AF-647) preadsorbed secondary antibody (Abcam). The sections were also labeled with Isolectin Griffonia simplicifolia AF-488 conjugate (Thermo Fisher, Waltham, MA) which binds d-galactosyl residues of galactose α−1,3 galactose (Gal α−1,3 Gal). Nuclei were labeled with 4′,6-diamidina-2-phenylindole (DAPI).
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