Dimethyl sulfoxide, apigenin, genistein, LPS (from an Escherichia coli strain), 2,7-dichlorofluorescein diacetate (DCF-DA), Griess reagent, and the Cell Proliferation Kit II (XTT) were obtained from Sigma (St. Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum, penicillin (100 IU/mL)-streptomycin (100 μg/mL), and trypsin-EDTA were purchased from Gibco (Grand Island, NY, USA). The ELISA kits for TNF-α and IL-6 were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Antibodies against p-ERK1/2, ERK1/2, p-SAPK/JNK, SAPK/JNK, iNOS, COX2, and β-actin, and secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). SNAH was obtained from ChemFaces Biochemical Co. Ltd. (Wuhan, China), and the purity of SNAH was ≥98%. It was dissolved at a concentration of 100 mM in Dimethyl sulfoxide. The IUPAC nomenclature of SNAH is (E)-3-(4-hydroxy-3,5-dimethoxyphenyl)acrylaldehyde, and the chemical structure was drawn using ChemDraw software (https://www.perkinelmer.com/category/chemdraw ).
Antibodies against p erk1 2
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against p-ERK1/2 are specific molecular probes used to detect the phosphorylated forms of the extracellular signal-regulated kinases 1 and 2 (ERK1/2) in biological samples. These antibodies recognize the dual phosphorylation of ERK1/2 at the Thr202/Tyr204 (ERK1) and Thr185/Tyr187 (ERK2) residues, which is a key event in the activation of the ERK signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
P jnk, by Cell Signaling Technology (2 mentions)
Apigenin, by Merck Group (1 mentions)
Genistein, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
P sapk jnk, by Cell Signaling Technology (1 mentions)
Cell proliferation kit 2, by Merck Group (1 mentions)
Elisa kits for tnf α and il 6, by R&D Systems (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Griess reagent, by Merck Group (1 mentions)
2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions)
Atenolol, by Merck Group (1 mentions)
Ici 118 551, by Merck Group (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Indometacin, by Merck Group (1 mentions)
Adipsin, by Santa Cruz Biotechnology (1 mentions)
Rosiglitazone, by Merck Group (1 mentions)
3 isobutylmethylxanthine, by Merck Group (1 mentions)
Oil red o, by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
6 protocols using antibodies against p erk1 2
1
Induction of Inflammatory Responses
2
Adrenoceptor Modulation in Cellular Assays
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Atenolol, ICI118551, NNK, and an anti-beta-actin antibody were purchased from Sigma (St. Louis, MO, USA). Antibodies against beta1- and beta2-adrenoceptors, cyclin D1, VEGF, and CD31 were purchased from Abcam (Cambridge, MA, USA). Antibodies against p-ERK1/2, Bcl-2, and Bax were purchased from Cell Signaling (Beverley, MA, USA). siRNAs targeting beta1- or beta2-adrenoceptor mRNAs and nonspecific negative control siRNA were obtained from Gene Pharma (Shanghai, China).
3
Adipogenic Differentiation Pathway Modulation
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PG, 3-isobutylmethylxanthine, dexamethasone, rosiglitazone, indometacin, insulin, methyl thiazolyltetrazolium (MTT), and Oil Red-O were purchased from Sigma-Aldrich (USA). Antibodies against CCAAT enhancer binding protein-α, peroxisome proliferator-activated receptor-activated receptor-γ, ERK2, adipsin, and aP2 antibodies were obtained from Santa Cruz Biotechnology (USA). Antibodies against p-ERK1/2, p-p38, and p-JNK were purchased from Cell Signaling Technology (USA). Actin antibody was purchased from MP Biomedicals (USA). Anti-mouse and anti-rabbit horseradish peroxidase-conjugated secondary antibodies were obtained from KPL (USA). PD98059 was purchased from Calbiochem (USA).
4
Antibody and Reagent Sources
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H2O2 and catalase were purchased from Sigma. Antibodies against p-ERK1/2, ERK1/2, p-Src Y419, p-Src Y530 and Src were obtained from Cell Signaling (Beverly, MA, USA). Anti-Csk, anti-p-p38, anti-p38, anti-p-JNK, anti-JNK antibodies were obtained from Santa Cruz Technology (Santa Cruz, CA, USA). Antibody against caveolin-1 was obtained from BD Biosciences (San Diego, CA, USA). Tubulin antibody was obtained from Sigma. All Horseradish peroxidase (HRP)-conjugated secondary antibodies for immunoblotting were obtained from Santa Cruz Technology. Alexa flour 568-conjugated rabbit secondary antibody was obtained from Life Technologies.
5
Investigating Molecular Mechanisms in Cancer
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5-FU was purchased from Calbiochem (San Diego, CA, USA). Curcumin, X-treme GENE™ HP DNA transfection reagent and antibody against vinculin were procured from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against p-ERK1/2, p-p38, p-JNK, p-Akt, p-IKK, c-Myc and caspases were obtained from Cell Signaling Technology (Beverly, MA, USA). Antibodies against p-p65, MDR-1, VEGF, Bcl-2, cIAP1, XIAP, survivin, TS, PARP, Cox-2 and GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against ABCG2 was purchased from Abcam (Cambridge, Mass, USA).
6
Antioxidant Activity and Signaling Assays
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All reagents for cell culture were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against p-ERK1/2, ERK1/2, MEK and U0126 were purchased from Cell signaling Inc. (Danvers, Mass, MA), collagen I (a1) and Fibronectin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA,Calif,USA), and NOX2 (gp91phox) was purchased from Abcam (Cambridge, UK). Anti-β-actin and a cytochrome c reductase (NADPH) assay kit were purchased from Sigma-Aldrich Ltd. (St. Louis, MO, USA). Dichlorodihydrofluorescein diacetate (DCFH-DA) was purchased from Invitrogen and the Oxiselect TM thiobarbituric acid reactive substances (TBARS) assay kit for malondialdehyde (MDA) quantification was purchased from Cell Biolabs, Inc. (San Diego, CA, USA).
The HRP-conjugated secondary antibodies and enhanced chemiluminescence (ECL) kit for Western blot were purchased from Amersham Biosciences (Piscataway, NJ). AKF-PD (Lot No.090601) was synthesized by Sunshine Lake Pharma Co, Ltd. (Shenzhen, Guangdong, China). Losartan was from Merck & Co, Inc (Whitehouse Station, NJ). All other chemicals were from Sigma-Aldrich Ltd unless otherwise indicated.
The HRP-conjugated secondary antibodies and enhanced chemiluminescence (ECL) kit for Western blot were purchased from Amersham Biosciences (Piscataway, NJ). AKF-PD (Lot No.090601) was synthesized by Sunshine Lake Pharma Co, Ltd. (Shenzhen, Guangdong, China). Losartan was from Merck & Co, Inc (Whitehouse Station, NJ). All other chemicals were from Sigma-Aldrich Ltd unless otherwise indicated.
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