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2 protocols using streptococcus suis cvcc 3928

1

Bacterial Strains and Cell Line Acquisition

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The bacterial strains S. aureus ATCC 43300, Staphylococcus epidermidis ATCC 12228, and Escherichia coli ATCC 25922 were purchased from American Type Culture Collection (ATCC). S. aureus CVCC 546, Streptococcus pneumoniae CVCC 2350, Streptococcus suis CVCC 3928, Salmonella pullorum CVCC 533, Salmonella enteritidis CVCC 3377, and E. coli K88 were purchased from the China Veterinary Culture Collection Center (CVCC). RAW 264.7 murine macrophages were obtained from Peking Union Medical College.
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2

Antimicrobial Activity of Peptides ID13 and DLP4

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The bacterial strains Staphylococcus epidermidis ATCC 12228 and Escherichia coli ATCC 25922 were purchased from American Type Culture Collection (ATCC). S. aureus CVCC 546, Streptococcus pneumoniae CVCC 2350, Streptococcus suis CVCC 3928, Salmonella pullorum CVCC 533, and Salmonella Enteritidis CVCC 3377 were purchased from the China Veterinary Culture Collection Center (CVCC). Mouse macrophages RAW 264.7 and endometrial epithelial cells (MEECs) were obtained from Peking Union Medical College and iCell Bioscience Inc. (Shanghai, China), respectively. LTA and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (China). Antibiotics used in the study were purchased from Meilun Biotech Co., Ltd (Dalian, China). Peptides ID13 and DLP4 were expressed and purified in our lab as previously described (Li et al. 2020 ), with the purity of 91.2% and 92%, respectively. Other reagents were of analytical grade.
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