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3 protocols using ethylene diamine

1

Synthesis of Aspartame Precursor

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l-Aspartyl-l-phenylalanine methyl ester was supplied by Ajinomoto Co., Inc. Triphenyl phosphite (P(OPh)3), l-phenylalanine (Phe), succinic acid, ethylene diamine and p-phenylenediamine were purchased from Tokyo Chemical Industry Co., Ltd. 5% Palladium on activated carbon (Pd/C) and N-methylpyrrolidone (NMP) were purchased from FUJIFILM Wako Pure Chemical Corporation. Dimethyl sulfoxide (DMSO), sulfuric acid, nitric acid, pyridine, ethanol, tetrahydrofuran, ethyl acetate, acetone and toluene were purchased from Kanto Chemical Co., Inc. All the chemicals were directly used as purchased.
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2

Fabrication of Biofunctional Textile Composites

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17 α-methyltestosterone (MT, powder, >98% purity), methacrylic acid (liquid, >99% purity), ethylene glycol dimethacrylate (liquid, ˃97% purity), and ethylenediamine (liquid, 98% purity) were purchased from Tokyo Chemical Industry Co., Ltd., Japan. Azobisisobutyronitrile (12 wt% in acetone) was purchased from Sigma Aldrich, USA. Citric acid anhydrous (solid, ACS reagent, ≥99.5% purity) and ethanol (liquid, ACS grade, >99% purity) were purchased from Merck, Germany. Acetonitrile (liquid, >97% purity) was purchased from Macron and Avantor, USA. Hydrogen peroxide (30%, AR grade) was purchased from QReC New Zealand, while sodium hydroxide (pellets, AR grade, >98.6%) was purchased from Fisher, India. An SPE cartridge (C18E 500 mg/30 mL) was purchased from Welch, USA. Cotton fabric, 220 threads per inch, was supplied from a local company in Thailand.
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3

Affinity-Based Monoclonal Antibody Purification

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Sodium dihydrogen phosphate, 2-hydrate, disodium hydrogen phosphate, anhydrous (Kanto Chemical Co., Inc., Tokyo, Japan), and water (Milli-Q system; Merck Millipore, Darmstadt, Germany) were used in preparing the mobile phase, 100 mM phosphate buffer (pH 7.0). Tetraethoxysilane (TEOS), 3-aminopropyltrimethoxysilane (APTES), 3-methacryloxypropyltrimethoxysilane (MPS), and 3-glycidyloxypropyltrimethoxysilane (GPTMS) were obtained from Shin-Etsu Chemical (Tokyo, Japan). Polyethyehylene oxide, Immunoglobulin G human, and Protein A were provided by Sigma-Aldrich Co. (St. Louis, MO, USA).
N-Disuccinimidyl carbonate (DSC), glycidyl methacrylate (GMA), 2-hydroxyethyl methacrylate (HEMA), N,N-dimethylformamide (DMF), D-glucitol, ethylene diamine, and 2,2′-azobisisobutyronitrile (AIBN) were obtained from Tokyo Chemical Industry Co. (Tokyo, Japan). A Chinese hamster ovary (CHO) cell culture medium containing monoclonal antibody was obtained from manufacturing technology association of pharmaceutical that asked for confidentiality. All other reagents were of analytical grade and were purchased from KISHIDA Chemicals (Tokyo, Japan).
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