Huxuc 03061

Manufactured by Cyagen

Sourced in China

The HUXUC-03061 is a laboratory instrument designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cells. The core function of this product is to regulate temperature, humidity, and gas composition to support optimal cell culture conditions.

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Lab products found in correlation

Hucmscs, by Cyagen (1 mentions)

2 protocols using huxuc 03061

Lentiviral Transduction of HUCMSCs

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HUCMSCs were purchased from Wuhan Hamilton Biotechnology Co., Ltd. HUCMSCs (4 × 10⁶) were seeded in a 6-well plate, and when cells covered 90% of every well, the medium (Cyagen, HUXUC-03061) was changed, and the lentivirus was added. The lentivirus was packaged previously, and it was added to generate HUCMSCs^Wnt10b. Empty vector-transduced HUCMSCs (HUCMSCs^Emp) were used as controls in the study. Cells were cultured with growth media (Cyagen, HUXUC-03061), and P5–P7 were used for experiments.

Liu Y., Fang J., Zhang Q., Zhang X., Cao Y., Chen W., Shao Z., Yang S., Wu D., Hung M., Zhang Y., Tong W, & Tian H. (2020). Wnt10b-overexpressing umbilical cord mesenchymal stem cells promote critical size rat calvarial defect healing by enhanced osteogenesis and VEGF-mediated angiogenesis. Journal of Orthopaedic Translation, 23, 29-37.

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Wnt10b Transduced Umbilical Cord MSCs

Cited 1 time

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HUCMSCs (human umbilical cord mesenchymal stem cells) were purchased from Wuhan Hamilton Biotechnology Co., LTD, and inoculated into 6-well plates at 4 × 10⁶ cells/well. When cells reached 90% confluence in each well, the culture medium (Huxuc-03061; Cyagen Biosciences Inc., Guangzhou, China) was changed and lentiviral vector was used to introduce the Wnt10b gene to construct transgenic umbilical cord mesenchymal stem cells (HUCMSCs^Wnt [10 (link)]b); umbilical cord mesenchymal stem cells transfected with empty vector (HUCMSCs^Emp) were used as controls. Both were provided by the technical department of Wuhan Hamilton Biotechnology Co., Ltd [14 ].

Hu Y., He Y., Fang J., Liu Y., Cao Y., Tong W., Chen W., Shao Z., Liu Y, & Tian H. (2022). Wnt10b-overexpressing umbilical cord mesenchymal stem cells promote fracture healing via accelerated cartilage callus to bone remodeling. Bioengineered, 13(4), 10313-10323.

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