Glutamine penicillin streptomycin solution

HEK293 and HeLa cells were purchased from National Centre for Cell Science, India and grown in DMEM (HiMedia) supplemented with 5% fetal bovine serum (HiMedia) and glutamine–penicillin–streptomycin solution (HiMedia). The cells were grown in incubator supplied with 5% CO₂. The plasmid constructs were transfected using standard calcium phosphate precipitation method. The siRNAs, siControl (siRNA Negative control, Cat. no. SR-CL000-005; Eurogentec), siFAM47E (SMARTpool - siGENOME siRNA targeting FAM47E, Cat. no. M185579-00-0005; Dharmacon), and siPRMT5 (5′-CUU UGA GAC UGU GCU UUA U 3′) were transfected using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific).

Jurkat and Raji cell lines were obtained from National Centre for Cell Science (NCCS), Pune, India. Cell line authentication via STR profiling was conducted by cell repository. Cells were propagated in IMDM (for Jurkat), RPMI-1640 (for Raji) media, supplemented with 10% heat-inactivated FBS, 2 mM Glutamine-Penicillin-Streptomycin solution (Hi-Media, Mumbai, India). Cells were maintained in a humidified incubator at 37°C with supply of 5% CO₂. Sub-culturing of Jurkat and Raji cells in suspension were performed when they reached around 70% confluency.