Goat anti il 23r

Manufactured by Abcam

Goat anti-IL-23R is a polyclonal antibody that recognizes the interleukin-23 receptor (IL-23R). IL-23R is a subunit of the IL-23 receptor complex, which is involved in the immune response. The antibody can be used in various immunological applications to detect and study IL-23R expression and function.

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Lab products found in correlation

Lsm 710, by Zeiss (1 mentions) Hochest 33342, by Beyotime (1 mentions) Confocor 3, by Zeiss (1 mentions) Facs calibur, by Miltenyi Biotec (1 mentions) Imagescope, by Leica (1 mentions) Rabbit anti gata3, by Abcam (1 mentions)

2 protocols using goat anti il 23r

Characterization of IL-23R and CD133 Expression

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For detection of IL-23R, cells were cultured with 1:100 diluted goat anti-IL-23R (Abcam) at 4° C and then with 1:100 diluted rabbit anti-goat PE-conjugated secondary antibody (ProteinTech Group, Wuhan, China) at 4° C in the dark. After that, half cells were analyzed by flow cytometer and half cells were further stained with hochest33342 (5 μg/ml, Beyotime) for 10min followed by observation under the fluorescence microscope (LSM 710 and ConfoCor 3, Zeiss, Germany).
For detection of CD133, saponin permeabilized cells were incubated with PE labeled anti-CD133 (Miltenyi Biotec, Germany) and then were analyzed by FACS Calibur.

Jiang Q., Sun Y., Guo Z., Chen R., Ma S., Fu M., Zhu H., Ning Q., Lei P, & Shen G. (2018). IL-23 enhances the malignant properties of hepatoma cells by attenuation of HNF4α. Oncotarget, 9(47), 28309-28321.

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Multiplex Immunohistochemistry for CD4, IL23R, and GATA3

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Multiplex sequential immunohistochemistry was performed with FFPE (5μm) tissue sections as we previously reported (Gunderson et al. 2016 (link)). Primary rat or rabbit antibodies were then serially stained for 1 hour at room temperature using rat anti-CD4 (4SM95, 1:50, eBioscience), Goat anti-IL23R (1:100, Abcam), and rabbit anti-GATA3 (EPR16651, 1:500, Abcam). Histofine Simple Stain MAX PO HRP conjugated polymer (Nichirei Biosciences Inc.) was utilized for detection followed by AEC for peroxidase detection. Multiplex images were coregistered using CellProfiler software (Broad Institute), deconvoluted using Image J, pseudocolored, and merged in ImageScope (Aperio, Leica). High magnification images were created with a 4x zoom from a 20x original magnification. Total positive cells were manually counted for each cross section, and the results were normalized to total tissue area.

Liu Y., Wang Z., De La Torre R., Barling A., Tsujikawa T., Hornick N., Hanifin J., Simpson E., Wang Y., Swanzey E., Wortham A., Ding H., Coussens L.M, & Kulesz-Martin M. (2016). Trim32 deficiency enhances Th2 immunity and predisposes to features of atopic dermatitis. The Journal of investigative dermatology, 137(2), 359-366.

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