Dansylsarcosine piperidinium salt

Phorbol-12-myristate-13-acetate (PMA), LPS, nigericin, ATP, and dansylsarcosine piperidinium salt (DSS) were purchased from Sigma-Aldrich. Caspase-1 inhibitor (VX-765) was purchased from Selleck. Recombinant human IL-1β protein (200-01B) and human IL-1RA (200-01RA) were obtained from Peprotech. Recombinant mouse IL-1β protein (401-ML/CF) and recombinant mouse IL-1RA (480-RM/CF) were purchased from R&D Systems. Trizol reagent was from Invitrogen, and Lipofectamine 2000 was from Invitrogen. The human IL-1β ELISA kit was purchased from BD Biosciences, the mouse IL-1β and tumor necrosis factor (TNF)-α ELISA kits were purchased from R&D Systems, and the LPS ELISA kit was purchased from Expandbio (Beijing, China).
Antibodies against NLRP3 (D4d8T), caspase-1 (D7F10), and IL-1β (D3U3E and 3A6) were purchased from Cell Signaling Technology. Antibody against DENV-NS3 (GTX124252) and DENV-Prm (GTX128092) were purchased from Genetex. Anti-β-actin antibody (66009) was purchased from Proteintech. Antibody against ASC (sc-271054) was purchased from Santa Cruz Biotechnology. Anti-mouse IgG Dylight 649, anti-mouse IgG Dylight 488, anti-rabbit IgG Dylight 649, and anti-rabbit IgG fluorescein isothiocyanate (FITC) were purchased from Abbkine.

LPS, ATP, TPA, and dansylsarcosine piperidinium salt (DSS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI-1640 and DMEM were obtained from Gibco (Grand Island, NY, USA). Nigericin and Ac-YVAD-cmk were obtained from InvivoGene Biotech Co., Ltd. (San Diego, CA, USA). The caspase-1 inhibitor VX-765 was purchased from Selleckchem (Houston, TX, USA). Antibody against Flag (F3165) (1:2000) and monoclonal mouse anti-GAPDH (G9295) (1:5000) were purchased from Sigma (St Louis, MO, USA). Monoclonal rabbit anti-NLRP3 (D2P5E) (1:1000), monoclonal rabbit anti-IL-1β (D3U3E) (1:1000), IL-1β mouse mAb (3A6) (1:1000), and monoclonal rabbit anti-caspase-1 (catalog no. 2225) (1:1000) were purchased from Cell Signaling Technology (Beverly, MA, USA). Monoclonal mouse anti-ASC (sc-271054) (1:500) and polyclonal rabbit anti-IL-1β (sc-7884) (1:500) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monoclonal mouse anti-NLRP3 (ALX-804-818) (1:500) was purchased from Enzo Life Sciences (Shanghai, China) to detect endogenous NLRP3 in THP1 cells by immunofluorescence microscopy. Lipofectamine 2000, normal rabbit immunoglobulin G (IgG), and normal mouse IgG were purchased from Invitrogen Corporation (Carlsbad, CA, USA).

Lipopolysaccharide (LPS), ATP, phorbol-12-myristate-13-acetate (TPA), and dansylsarcosine piperidinium salt (DSS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI 1640 and Dulbecco modified Eagle medium (DMEM) were obtained from Gibco (Grand Island, NY, USA). Nigericin was obtained from InvivoGene Biotech Co., Ltd. (San Diego, CA, USA). Antibody against Flag (F3165) and monoclonal mouse anti-GAPDH (G9295) were purchased from Sigma. Monoclonal rabbit anti-NLRP3 (D2P5E), monoclonal rabbit anti-IL-1β (D3U3E), monoclonal rabbit anti-caspase-1 (catalog no. 2225) and monoclonal rabbit anti-eIF4G (C45A4) were purchased from Cell Signaling Technology (Beverly, MA, USA). Monoclonal mouse anti-ASC (sc-271054) and polyclonal rabbit anti-IL-1β (sc-7884) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monoclonal mouse anti-NLRP3 (ALX-804-818) was purchased from Enzo Life Sciences (Shang Hai, China) to detection endogenous NLRP3 in THP1 cells by immunofluorescence microscopy. Polyclonal rabbit anti-3D antibody was produced by ABclonal Technology (Wuhan, China). Lipofectamine 2000, normal rabbit IgG and normal mouse IgG were purchased from Invitrogen Corporation (CA, USA).