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Fp 8500 spectrophotometer

Manufactured by Jasco

The FP-8500 spectrophotometer is a versatile laboratory instrument designed for absorbance and transmittance measurements across a wide range of wavelengths. It is capable of performing accurate and reliable spectroscopic analysis for various applications in scientific research and testing.

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4 protocols using fp 8500 spectrophotometer

1

Characterization of Organic Polymers

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Melting points were measured by a Büchi M-560 apparatus. HRMS spectra were recorded on a Bruker ESI microTOF II instrument. 1H, 13C, 2D-COSY, and -HMQC NMR spectra (400 or 100 MHz) were measured in CDCl3 containing tetramethylsilane (TMS) as an internal standard with an ECX400 NMR spectrometer. UV/vis spectra were measured in a quartz cell (1 cm path length) by recording on a JASCO V-650 spectrometer equipped with an ETCS-761 temperature controller. Fluorescence spectra were measured in the same cell using a JASCO FP-8500 spectrophotometer equipped with an ETC-815 temperature controller. Fluorescence lifetime decays were observed in the same cell by a Hamamatsu Quantaurus-Tau single-photon-counting instrument fitted with an LED excitation light (340 nm). IR spectra were measured on a JASCO FT/IR-4700 spectrometer. The molecular weights of the PTs were determined using polystyrene standards through an analytical GPC with a TOSOH TSKgel α-4000 column (condition: 40 °C, 0.5 mL min−1, DMF).
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2

Spectroscopic Characterization of AuNCs

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Fluorescence
spectral
data were monitored by a JASCO FP-8500 spectrophotometer attached
with 150-W xenon light as an excitation resource. A double-beam JASCO
V-630 UV–visible spectrophotometer was applied for the recording
of the absorption spectral analysis. HR-TEM images were achieved on
JEOL JEM 2100 at an accelerating voltage of 200 kV. For HR-TEM analysis,
the samples were equipped by dipping OVA–AuNCs on the carbon-coated
copper grid. The sample was investigated by HR-TEM images after drying.
Time-resolved fluorescence decay investigation was achieved on a HORIBA
Scientific time-correlated single-photon counting unit equipped with
a 390 nm light source as an excitation. CD studies were tracked in
the range from 200 to 260 nm with a scan speed of 50 nm min–1 using a JASCO-810 spectropolarimeter equipped with a quartz cuvette
of 0.1 cm path length.
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3

Peptide Fluorescence Spectroscopy Protocol

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Peptide samples were prepared at a concentration of 1.0 mM in PBS at pH 7.4. Measurements were collected on a Jasco FP-8500 Spectrophotometer. Samples were excited at 270 nm, and the emission was collected from 280–380 nm at a scanning speed of 200 nm/min. Excitation and emission bandwidth were 5 nm and 2.5 nm, respectively.
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4

Synthesis and Characterization of Hydrazone and Azine Derivatives

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4-Methoxysalicylaldehyde hydrazone (2),20 (link) 1,3,5-triformylphloroglucinol (3),18 (link) tris(N-4-methoxysalicylideneaniline) (4),18 (link) and 4-methoxysalicylaldehyde azine (5)16b were prepared according to procedures reported previously. All solvents and chemicals were obtained from commercial sources and used without further purification. 1H and 13C NMR spectra were recorded using JEOL ECA-600II spectrometer with DMSO-d5 (2.49 ppm for 1H NMR) and DMSO-d6 (39.5 ppm for 13C NMR) as internal references. Mass spectra were recorded using a JEOL JMS-700T spectrometer. Infrared (IR) spectra were recorded using a JASCO FT/IR-4100 spectrometer. UV-vis absorption spectra were recorded using a HITACHI U-3900H spectrometer. Fluorescence spectra were recorded using a JASCO FP-8500 spectrophotometer.
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