Bendroflumethiazide

Atenolol, bendroflumethiazide, carbamazepine, diclofenac, and furosemide were obtained from Sigma-Aldrich (Steinheim, Germany). Oxazepam was obtained from Apoteket (Kristianstad, Sverige). Stock solutions of the individual pharmaceuticals in methanol were prepared at a concentration of 10 mg L⁻¹ and stored at −18 °C. Deuterated internal standards (ISs), d₁₀-carbamazepine and d₇-Atenolol, were obtained from CDN Isotopes (Pointe-Claire, Quebec, Canada). The purity of all analytical standards was >95 % and deuterated purity was >98 %. Ammonium acetate (98 %) was obtained from KMF (St. Augustin, Germany) and acetic acid (99–100 %), aqueous ammonia solution, heptane, ethyl acetate, methanol, and acetone from Merck (Darmstadt, Germany). Ethylenediaminetriacetic acid (EDTA) was obtained from Sigma-Aldrich (>98 %). Ammonium formate, formic acid, acetonitrile, and water were purchased from Merck.

BACE inhibitor AZD3839, etodolac, fenoprofen, and pargyline were supplied by Selleckchem. Dexketoprofen and penbutolol were from Medchemexpress. Bendroflumethiazide was obtained from Sigma-Aldrich.

Compounds were obtained from the following sources: 2-chlorobenzenesulfonamide, althiazide, bendroflumethiazide, benzthiazide, chlorothiazide, clopamide, cyclothiazide, dichlorofenamide, hydroflumethiazide, indapamide, metalozone, polythiazide, trichlormethiazide and xipamide were obtained from Sigma-Aldrich; chlorthalidone, clorexolone, fenquizone, and quinethazone were obtained from SantaCruz Biotechnology; hydrochlorothiazide and furosemide were obtained from AlfaAesar. Compound stock solutions (10–50) mM were prepared in dimethylsulfoxide (DMSO) and stored in a dark box at +4°C. Marvin was used for drawing, displaying and characterizing chemical structures, substructures and reactions; Marvin 19.4.0, 2019, ChemAxon (http://www.chemaxon.com).