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2 protocols using ethylenediaminetetraacetic dianhydride

1

Functionalization of Poultry Feathers

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Poultry feathers were supplied by Paviso Alimentación (Sevilla, Spain). Succinic anhydride (SA) and ethylenediaminetetraacetic dianhydride (EDTAD), used as functionalization reagents, were purchased from Sigma Aldrich (St. Louis, MO, USA). Other reagents, i.e., hydrochloric acid (HCl) or sodium hydroxide (NaOH), were purchased from Panreac Química, S.A. (Barcelona, Spain). All the reagents were of analytical grade and used without purification. Defibronated sheep blood was purchased from Håtunalab AB (Bro, Sweden).
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2

Reagents for Protein Characterization

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Guanidine
hydrochloride (GuHCl), bovine pancreatic
ribonuclease A (RNase A), bovine pancreatic insulin, thioflavin T,
ethylenediaminetetraacetic dianhydride, 1,3,5 benzenetricarbony trichloride,
and 3,3′,4,4′-biphenyltetracarboxylic dianhydride were
purchased from Sigma-Aldrich. 5,5′-Dithiobis-2-nitrobenzoic
acid (DTNB), dithiothreitol (DTT), and tris(2-carboxyethyl) phosphine
hydrochloride (TCEP) were obtained from Gold Biotechnology. Size-exclusion
PD-10 columns were purchased from GE Healthcare, and Congo Red was
purchased from MP Biomedicals.
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