Cryo-embedded liver samples were sectioned (10 µm) and stained with primary antibodies against EGF-like module-containing mucin-like hormone receptor-like 1 (F4/80; total macrophages; USBiological Life Sciences, Salem, MA USA; 1:250), integrin, alpha X (CD11c; M1, pro-inflammatory macrophages; Invitrogen; 1:300), and mannose receptor (CD206; M2, anti-inflammatory macrophages; Cell Signaling; 1:500) as previously described46 (link),77 (link). Secondary antibodies used included goat anti-Armenian hamster IgG Alexa Fluor 594 (Jackson ImmunoResearch Laboratories, West Grove, PA, USA; 1:500), goat anti-chicken IgG Alexa Fluor 647 (Jackson ImmunoResearch Laboratories; 1:500), and goat anti-rabbit IgG Alexa Fluor 488 (Jackson ImmunoResearch Laboratories; 1:500). Sections were mounted in Prolong Diamond Mounting Medium with DAPI (Abcam) and images were acquired using a Leica 3D Thunder scope from three non-intersecting fields per mouse. Intensity of fluorescence was measured as percent of total area using Image J after each image had its background intensity subtracted out.
Goat anti armenian hamster igg alexa fluor 594
Manufactured by Jackson ImmunoResearch
Sourced in United States
Goat anti-Armenian hamster IgG Alexa Fluor 594 is a secondary antibody used in immunological applications. It is produced by immunizing goats with Armenian hamster IgG and conjugating the resulting antibodies with the Alexa Fluor 594 fluorescent dye.
Automatically generated - may contain errors
2 protocols using goat anti armenian hamster igg alexa fluor 594
1
Macrophage Phenotyping in Liver Samples
2
Macrophage Phenotyping in Cryo-Embedded Liver
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Cryo-embedded liver samples were sectioned (10 μm) and stained with primary antibodies against EGF-like module-containing mucin-like hormone receptor-like 1 (F4/80; total macrophages; USBiological Life Sciences, Salem, MA USA; 1:250), integrin, alpha X (CD11c; M1, pro-inflammatory macrophages; Invitrogen; 1:300), and mannose receptor (CD206; M2, anti-inflammatory macrophages; Cell Signaling; 1:500) as previously described46 (link),76 (link). Secondary antibodies used included goat anti-Armenian hamster IgG Alexa Fluor 594 (Jackson ImmunoResearch Laboratories, West Grove, PA, USA; 1:500), goat anti-chicken IgG Alexa Fluor 647 (Jackson ImmunoResearch Laboratories; 1:500), and goat anti-rabbit IgG Alexa Fluor 488 (Jackson ImmunoResearch Laboratories; 1:500). Sections were mounted in Prolong Diamond Mounting Medium with DAPI (Abcam) and images were acquired using a Leica 3D Thunder scope from three non-intersecting fields per mouse. Intensity of fluorescence was measured as percent of total area using Image J after each image had its background intensity subtracted out.
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