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Ventana discovery xt automation

Manufactured by Roche
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The Ventana Discovery XT Automation system is a high-throughput, automated slide processing platform designed for immunohistochemistry (IHC) and in situ hybridization (ISH) assays. The system is capable of performing various slide preparation steps, including deparaffinization, rehydration, antigen retrieval, and staining, in a fully automated manner. The Ventana Discovery XT Automation system is intended to streamline and standardize the workflow for clinical diagnostic and research laboratories.

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Discovery XT (190 citations) Ventana Discovery XT (183 citations) Ventana Discovery (31 citations)

2 protocols using ventana discovery xt automation

1

Pharmacodynamic Profiling of Sunvozertinib in PDX

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Tumor tissues from the LU3075 PDX mice model were collected at 2, 8, 24 hours on day 28 post last dosing of sunvozertinib. Samples were harvested following formalin fixation and paraffin embedding (FFPE) for further study. IHC was performed on 3 μm FFPE sections using a Lab Vision autostainer (Thermo) for pERK (Thr202/Tyr204) staining, and Ventana discovery XT automation (Roche) for pEGFR (Tyr1068) staining. Monoclonal rabbit anti-pEGFR (Tyr1068) antibody (Cat.2234) and pERK (Thr202/Tyr204) antibody (Cat.4376) were purchased from Cell Signaling Technology. The stained IHC slides were firstly reviewed and interpreted by a qualified pathologist and then quantified by HALO system. “H” score was also performed when the cases were illegible on HALO platform. Statistical analysis was performed by t-test.
Wang M., Yang J.C., Mitchell P.L., Fang J., Camidge D.R., Nian W., Chiu C.H., Zhou J., Zhao Y., Su W.C., Yang T.Y., Zhu V.W., Millward M., Fan Y., Huang W.T., Cheng Y., Jiang L., Brungs D., Bazhenova L., Lee C.K., Gao B., Xu Y., Hsu W.H., Zheng L, & Jänne P.A. (2022). Sunvozertinib, a Selective EGFR Inhibitor for Previously Treated Non–Small Cell Lung Cancer with EGFR Exon 20 Insertion Mutations. Cancer Discovery, 12(7), 1676-1689.
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2

Temporal Regulation of EGFR and ERK Activation in PDX Model

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Tumor tissues from the LU3075 PDX mice model were collected at 2, 8, and 24 hours on day 28 after the last dosing of sunvozertinib. Samples were harvested following formalin fixation and paraffin embedding (FFPE) for further study. IHC was performed on 3-μm FFPE sections using a Lab Vision autostainer (Thermo) for pERK (Thr202/Tyr204) staining and Ventana discovery XT automation (Roche) for pEGFR (Tyr1068) staining. Monoclonal rabbit anti-pEGFR (Tyr1068) antibody (cat. #2234) and pERK (Thr202/Tyr204) antibody (cat. #4376) were purchased from Cell Signaling Technology. The stained IHC slides were first reviewed and interpreted by a qualified pathologist and then quantified by the HALO system. H-score was also performed when the cases were illegible on the HALO platform. Statistical analysis was performed by a t test.
Wang M., Yang J.C., Mitchell P.L., Fang J., Camidge D.R., Nian W., Chiu C.H., Zhou J., Zhao Y., Su W.C., Yang T.Y., Zhu V.W., Millward M., Fan Y., Huang W.T., Cheng Y., Jiang L., Brungs D., Bazhenova L., Lee C.K., Gao B., Xu Y., Hsu W.H., Zheng L, & Jänne P.A. (2022). Sunvozertinib, a Selective EGFR Inhibitor for Previously Treated Non–Small Cell Lung Cancer with EGFR Exon 20 Insertion Mutations. Cancer Discovery, 12(7), 1676-1689.
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