Engineered cardiac tissues were dissociated into single cells after 4 weeks of cultivation using activated papain solution containing 20 unit/ml papain (~15 min at 37 °C with gentle tapping), as described above for electrophysiological recordings. The enzyme reaction was terminated by FBS (10%) in DMEM/F-12 culture media. The dissociated hiPS-CMs were plated into XF96 Culture Plates (Seahorse Bioscience) coated with Matrigel (Corning, #354230) and cultured for 3 days. Subsequently, the plated hiPS-CMs were assayed in real-time using an XF-96 Extracellular Flux Analyzer (Seahorse Biosciences) for oxygen consumption rate (OCR), and extracellular acidification rate (ECAR) per the manufacturer’s protocols.
Xf96 culture plates
Manufactured by Agilent Technologies
The XF96 Culture Plates are a laboratory equipment product designed for cell culture applications. They provide a standardized 96-well format for conducting experiments and analyses. The plates are constructed to support cell growth and enable various measurements and observations during the culturing process.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using xf96 culture plates
1
Metabolic Analysis of hiPS-CMs
2
Metabolic Analysis of hiPS-CMs
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