HDFs and CAFs were treated with different inhibitors or agonists in complete DMEM media containing 10% FBS and 1% antibiotics for a duration of 48-72 hours as specified in figure legends. HDFs were treated with the AR inhibitor AZD3514 (10 μM, Adooq Biosciences, Cat# A12396), the PPP1 inhibitor Tautomycin (10 μM, SantaCruz, Cat# sc-507214) or DMSO as solvent control. CAFs were treated with the AR agonist Ostarine (Selleckchem, Cat# S1174) or DMSO. Furthermore, HDFs were cultured in DMEM supplemented with charcoal-treated FBS for 48 hours prior to treatment with DHT (10 nM, Sigma-Aldrich, Cat# 10300), the AR degrading PROTAC ARCC4 (1 μM, Tocris, Cat# 7254), or ethanol, and treatment was continued for the specified duration.
Ostarine
Manufactured by Selleck Chemicals
Ostarine is a selective androgen receptor modulator (SARM) that is primarily used in research laboratories. It is designed to have tissue-selective anabolic effects, with the goal of promoting muscle growth and development while minimizing the side effects associated with traditional anabolic steroids. Ostarine's core function is to selectively bind to and activate androgen receptors in target tissues.
Automatically generated - may contain errors
Lab products found in correlation
Tautomycin, by Santa Cruz Biotechnology (1 mentions)
Anti myogenin, by Santa Cruz Biotechnology (1 mentions)
Anti vinculin, by Santa Cruz Biotechnology (1 mentions)
Cyproterone acetate, by Santa Cruz Biotechnology (1 mentions)
Media and supplements, by Corning (1 mentions)
Anti myh, by Santa Cruz Biotechnology (1 mentions)
Anti erk1 2, by Santa Cruz Biotechnology (1 mentions)
Anti myod, by Santa Cruz Biotechnology (1 mentions)
Anti phospho erk1 2, by Santa Cruz Biotechnology (1 mentions)
Enzalutamide, by Selleck Chemicals (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
2 protocols using ostarine
1
Modulation of Fibroblast Signaling
2
Myogenic Differentiation Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Media and supplements were from Corning (Tewksbury, MA, USA). Ostarine (purity 99.36%) was purchased from Selleck Chemicals (No. S1174). Antibodies were as follows: anti-myogenin, anti-myh, anti-myoD, anti-vinculin, anti-phospho-ERK1/2, and anti-ERK1/2 were from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-GAPDH and all secondary antibodies for Western blot were from Sigma-Aldrich (Taufkirchen, Germany). The AR blockers used were as follows: enzalutamide was from Selleck Chemicals (No. S1250), and cyproterone acetate was from Santa Cruz Biotechnology (sc-204703). Unless otherwise stated, all other reagents were from Sigma-Aldrich.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!