Anti cd27 pe cy5

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-CD27 PE-Cy5 is a fluorescently-labeled antibody that binds to the CD27 cell surface receptor. CD27 is expressed on various immune cell types and is involved in cellular activation and proliferation. The PE-Cy5 conjugate provides a specific fluorescent signal that can be detected and quantified using flow cytometry or other compatible instrumentation.

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Lab products found in correlation

Anti ccr7 apc, by Thermo Fisher Scientific (1 mentions) Anti cd45ra pe cy7, by BD (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions) Anti cd45ro fitc, by BD (1 mentions) Anti cd19 pe, by Thermo Fisher Scientific (1 mentions) Anti cd38 apc, by Thermo Fisher Scientific (1 mentions) Anti cd24 fitc, by Thermo Fisher Scientific (1 mentions) Facsverse, by BD (1 mentions)

2 protocols using anti cd27 pe cy5

Identifying Subsets of T Cells

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The negatively sorted
CD69⁻CD25⁻HLA-DR⁻blast and non-blasts were stained with anti-CD45RO-FITC, anti-CD45RA-PE-Cy7 (BD
Biosciences), anti-CCR7-APC, eFluor-780, anti-CD27 PE-Cy5 (all eBioscience) and
anti-CD4-PE-Texas Red (Invitrogen), and analyzed using a flow cytometer
(LSRFortessa™, BD Biosciences).

MOSO M.A., ANDERSON J.L., ADIKARI S., GRAY L.R., KHOURY G., CHANG J.J., JACOBSON J.C., ELLETT A.M., CHENG W.J., SALEH S., ZAUNDERS J.J., PURCELL D.F., CAMERON P.U., CHURCHILL M.J., LEWIN S.R, & LU H.K. (2019). HIV latency can be established in proliferating and non-proliferating resting CD4+ T cells in vitro: implications for latency reversal. AIDS (London, England), 33(2), 199-209.

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Phenotypic Characterization of B Cell Subsets

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Peripheral venous whole blood was collected from subjects, and 0.83% ammonium
chloride was used to separate red blood cells. Whole blood was then stained with
the following fluorescent antibodies: anti-CD24-FITC, anti-CD19-PE,
anti-CD27-PEcy5, and anti-CD38-APC (eBioscience, San Diego, CA, USA).
Fluorescently-stained cells were then detected by BD FACSVerse flow cytometry
(BD Biosciences, San Jose, CA, USA). Peripheral venous blood B cells
(CD19⁺ lymphocytes) were divided into Breg cells
(CD19⁺CD24^hiCD38^hi), memory B cells
(CD19⁺CD27⁺CD24^hi), and plasma cells
(CD19⁺CD27^hiCD38^hi).

Guo S., Chen Q., Liang X., Mu M., He J., Fang Q., Song C, & Sang D. (2018). Reduced peripheral blood regulatory B cell levels are not associated with the Expanded Disability Status Scale score in multiple sclerosis. The Journal of International Medical Research, 46(9), 3970-3978.

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