Log In Sign up
The largest database of trusted experimental protocols

Rabbit polyclonal anti nr1 and nr2a antibody

Manufactured by Cell Signaling Technology
Visit Supplier

Rabbit polyclonal anti-NR1 and NR2A antibody is a laboratory reagent used to detect the presence and distribution of NR1 and NR2A subunits of the N-methyl-D-aspartate (NMDA) receptor in various biological samples. This antibody is generated by immunizing rabbits with synthetic peptides corresponding to specific regions of the NR1 and NR2A proteins.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit polyclonal anti phospho drp1 at ser616 antibody, by Cell Signaling Technology (2 mentions) Mouse monoclonal anti actin antibody, by Merck Group (2 mentions) Mouse monoclonal anti drp1 antibody, by BD (2 mentions)

Close spelling variants of this product

Anti-NR2A (4 citations) Anti-NR1 (3 citations) Anti-NR2A antibody (2 citations) Polyclonal rabbit antibody (2 citations)

2 protocols using rabbit polyclonal anti nr1 and nr2a antibody

1

Western Blot Analysis of ONH Astrocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured human ONH astrocytes were lysed with lysis buffer as described previously (Noh et al., 2013 (link)). The primary antibodies included rabbit polyclonal anti-NR1 and NR2A antibody (1:1,000; Cell Signaling Technology, Danvers, MA), rabbit polyclonal anti-GLAST (EAAT1, 1:500; Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal anti-DRP1 antibody (1:1,000; BD Transduction Laboratories), rabbit polyclonal anti-phospho-DRP1 at Ser616 antibody (1:1,000; Cell Signaling), and mouse monoclonal anti-actin antibody (1:5,000; Millipore). The scanned film images were analyzed with ImageJ (National Institute of Health, MD) and band densities were normalized to the band densities for actin.
Ju W.K., Kim K.Y., Noh Y.H., Hoshijima M., Lukas T.J., Ellisman M.H., Weinreb R.N, & Perkins G.A. (2014). Increased Mitochondrial Fission and Volume Density by Blocking Glutamate Excitotoxicity Protect Glaucomatous Optic Nerve Head Astrocytes. Glia, 63(5), 736-753.
+ Open protocol
+ Expand
2

Quantifying Astrocyte Protein Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured human ONH astrocytes were lysed with lysis buffer as described previously (Noh et al., 2013 (link)). The primary antibodies included rabbit polyclonal anti-NR1 and NR2A antibody (1:1000; Cell Signaling Technology, Inc., Danvers, MA), rabbit polyclonal anti-GLAST (EAAT1, 1:500; Santa Cruz Biotechnology Inc., Santa Cruz, CA), mouse monoclonal anti-DRP1 antibody (1:1000; BD Transduction Laboratories), rabbit polyclonal anti-phospho-DRP1 at Ser616 antibody (1:1000; Cell Signaling) and mouse monoclonal anti-actin antibody (1:5000; Millipore). The scanned film images were analyzed with ImageJ (National Institute of Health, MD, USA) and band densities were normalized to the band densities for actin.
Ju W.K., Kim K.Y., Noh Y.H., Hoshijima M., Lukas T.J., Ellisman M.H., Weinreb R.N, & Perkins G.A. (2014). Increased Mitochondrial Fission and Volume Density by Blocking Glutamate Excitotoxicity Protect Glaucomatous Optic Nerve Head Astrocytes. Glia, 63(5), 736-753.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!