The cells were cultured in 8-well chamber slides and the expression of protein biomarkers was determined using immunostaining following our previous protocols [9] (link). Primary antibodies (1:400 dilution) against TLR2 (ab213676), TLR4 (ab13556), HMGB1 (ab11354), RAGE (ab37647), ASC (ab175449), NLRP3 (ab214185), Caspase-1 (ab74279), IL-1β (ab156791), TREM1 (ab200729), NF-κB (ab86299) and IL-18 (ab106939) were used for staining and respective uorochrome conjugated secondary antibodies (1:400) were used for detection. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) and imaged using a uorescent slide scanner microscope (Leica Thunder, Germany). The experiments were performed with a negative control for xing the exposure time and to minimize the background. The mean uorescence intensity (MFI) normalized with the number of nuclei was used to calculate the log 2 fold-change (FC) following our previously reported protocol [10] (link).
Uorescent slide scanner microscope
Manufactured by Leica
Sourced in Germany
The Uorescent slide scanner microscope is a high-performance imaging system designed for analyzing fluorescent samples. It captures high-resolution digital images of slides or samples with exceptional clarity and precision. The core function of this microscope is to provide accurate and reliable imaging of fluorescent specimens for scientific and research applications.
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Lab products found in correlation
2 protocols using uorescent slide scanner microscope
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Immunostaining Quantification of Inflammatory Markers
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Immunostaining Quantification of Inflammatory Markers
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The cells were cultured in 8-well chamber slides and the expression of protein biomarkers was determined using immunostaining following our previous protocols [9] (link). Primary antibodies (1:400 dilution) against TLR2 (ab213676), TLR4 (ab13556), HMGB1 (ab11354), RAGE (ab37647), ASC (ab175449), NLRP3 (ab214185), Caspase-1 (ab74279), IL-1β (ab156791), TREM1 (ab200729), NF-κB (ab86299) and IL-18 (ab106939) were used for staining and respective uorochrome conjugated secondary antibodies (1:400) were used for detection. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) and imaged using a uorescent slide scanner microscope (Leica Thunder, Germany). The experiments were performed with a negative control for xing the exposure time and to minimize the background. The mean uorescence intensity (MFI) normalized with the number of nuclei was used to calculate the log 2 fold-change (FC) following our previously reported protocol [10] (link).
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