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Annexinv fitc dye

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AnnexinV-FITC is a fluorescently labeled protein used to detect apoptosis. It binds to phosphatidylserine, a membrane component that is translocated to the outer leaflet of the plasma membrane during the early stages of apoptosis.

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Lab products found in correlation

Pi dye, by BioLegend (2 mentions) Cellometer vision, by Revvity (2 mentions)

2 protocols using annexinv fitc dye

1

Annexin V-FITC Apoptosis Assay

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HaCaT cells were plated at 300,000 cells per well in a 6-well plate for 24 h, and incubated with increasing concentrations of either salpn or DMSO alone for 72 h. Both live and dead cells were collected by centrifugation at 2000 rpm, 4 °C for 5 min. The cell pellets were then resuspended in PBS, washed twice, and resuspended in 100 μl working solution (10 μl of 10 × Binding Buffer, 7 μl AnnexinV-FITC dye, 5 μl PI dye (BioLegend), and 78 μl dH2O). Samples were stained with the Annexin V working solution to distinguish cells that were alive (AnnexinV-; PI-), undergoing early primary apoptosis (AnnexinV+; PI-), and those undergoing late apoptosis/secondary necrosis (AnnexinV+; PI+). The percentage of cells in early or late apoptosis was measured by fluorescence performed on a Cellometer Vision cytofluorometer (Nexcelom). Data shown in each figure represent the mean ± SD of each set of triplicate salpn-treated or untreated cells from a representative experiment.
Issa N.T., Wathieu H., Glasgow E., Peran I., Parasido E., Li T., Simbulan-Rosenthal C.M., Rosenthal D., Medvedev A.V., Makarov S.S., Albanese C., Byers S.W, & Dakshanamurthy S. (2022). A novel chemo-phenotypic method identifies mixtures of salpn, vitamin D3, and pesticides involved in the development of colorectal and pancreatic cancer. Ecotoxicology and environmental safety, 233, 113330.
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2

Annexin V-FITC Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
HaCaT cells were plated at 300,000 cells per well in a 6-well plate for 24 h, and incubated with increasing concentrations of either salpn or DMSO alone for 72 h. Both live and dead cells were collected by centrifugation at 2000 rpm, 4 °C for 5 min. The cell pellets were then resuspended in PBS, washed twice, and resuspended in 100 μl working solution (10 μl of 10 × Binding Buffer, 7 μl AnnexinV-FITC dye, 5 μl PI dye (BioLegend), and 78 μl dH2O). Samples were stained with the Annexin V working solution to distinguish cells that were alive (AnnexinV-; PI-), undergoing early primary apoptosis (AnnexinV+; PI-), and those undergoing late apoptosis/secondary necrosis (AnnexinV+; PI+). The percentage of cells in early or late apoptosis was measured by fluorescence performed on a Cellometer Vision cytofluorometer (Nexcelom). Data shown in each figure represent the mean ± SD of each set of triplicate salpn-treated or untreated cells from a representative experiment.
Issa N.T., Wathieu H., Glasgow E., Peran I., Parasido E., Li T., Simbulan-Rosenthal C.M., Rosenthal D., Medvedev A.V., Makarov S.S., Albanese C., Byers S.W, & Dakshanamurthy S. (2022). A novel chemo-phenotypic method identifies mixtures of salpn, vitamin D3, and pesticides involved in the development of colorectal and pancreatic cancer. Ecotoxicology and environmental safety, 233, 113330.
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+ Expand

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