Serum cross laps ctx 1 elisa

Blood was collected by heart puncture and was then centrifuged at 5,000 rpm for 12 min to separate the plasma, which was further used for measurements of bone turnover products. Bone degradation product C-terminal telopeptide of type I collagen (CTX-I) was measured using serum cross-laps (CTX-I) ELISA (Immunodiagnostic Systems), while bone formation product procollagen type I N-propeptide (P1NP) ELISA was measured using Rat/Mouse P1NP EIA (Immunodiagnostic Systems), both following the manufacturer’s instructions.

Blood was collected from the orbital vein to non-heparinized tubes and let to clot and spun at high speed to collect serum. Serum levels of SAA3, TNFα and CTX-I were measured using the mouse SAA-3 ELISA kit (Sigma-Aldrich), LEGEND MAX™ Mouse TNF-α ELISA Kit (BioLegend) and the RatLapsTM EIA CTX-I (Immunodiagnostic Systems, Denmark), following the manufacturer’s instruction.
Human peripheral blood was collected by venous puncture and plasma was recovered. SAA and CTX-I were measured using the human SAA 1 and 2 ELISA (Sigma-Aldrich) and Serum Crosslaps (CTX-I) ELISA (ImmunoDiagnosticSystems, Denmark), following the manufacturer’s instruction. Circulating levels of cytokines/chemokines and bone biomarkers were determined using the Human Cytokine/Chemokine Panel A 48-Plex Discovery Assay^® Array (HD48A) and Human Bone 13-Plex Discovery Assay^® Array (HDBN13), respectively (Eve Technologies, Calgary, Canada).