Restore western blot stripping reagent

Cell culture reagents were purchased from Life Technologies. SP600125 (1,9-Pyrazoloanthrone), MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-tetrazolium-bromide), Monoclonal anti-β-Catenin antibody 6F9, Monoclonal Anti-α-Tubulin clone DM1A, Monoclonal Anti-Acetylated Tubulin Clone 6-11B-1 were purchased from Sigma-Aldrich. p53 antibody, Phospho-p53 (Ser46) antibody, p53 (1C12) Mouse mAb, p21 Waf1/Cip1 (DCS60) Mouse mAb, p21 Waf1/Cip1 (12D1) Rabbit mAb, Acetyl-p53 (Lys382) antibody, Phospho-p53 (Ser15), antibody, Phospho-Histone H2A.X 20E3 antibody, HDAC6 D2E5 antibody and Acetyl- β-Catenin (Lys49) antibody were purchased from Cell Signaling Technologies. ProLong Gold Antifade Reagent with DAPI, Wheat Germ Agglutinin Alexa-Fluor 594 Conjugate, Alexa-fluor conjugated and HRP conjugated secondary antibodies were purchased from Life Technologies. Purified Mouse Anti-Actin Ab-5 was purchased from BD Biosciences. Ischemin (MS120) and P-MYPT antibody were purchased from Merk Millipore. Phospho-Histone H3 pSer10 Antibody, TPPP/p25 antibody, TPPP antibody, Restore Western Blot Stripping reagent were purchased from Thermo Scientific. Protein G sepharose was purchased from GE Healthcare Life Sciences.

Cell culture reagents, Restore Western Blot Stripping reagent, monoclonal anti-GAPDH antibody and HRP conjugated secondary antibodies were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA). SP600125 (1,9-Pyrazoloanthrone), DMSO, Ponceau S, collagenase type I, monoclonal anti-β-Catenin and anti-α-Tubulin antibodies were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Fibroblast growth factor (bFGF) and epidermal growth factor (EGF) were purchased from PeproTech EC (London, UK). Monoclonal anti-POU5F1/OCT4 antibody was purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA); polyclonal anti-NANOG antibody from ReproCELL Inc. (Yokohama, Kanagawa, Japan), monoclonal anti-TTF1, TG, CD31, CD34 and CD45 antibodies from Cell Marque (Rocklin, CA, USA), and monoclonal anti-Actin Ab-5 from BD Italia, (Milan, Italy). Protease inhibitors cocktail tablets were purchased from Roche (Basel, Switzerland).