Bc43 spinning disk confocal system

All slides were scanned using the Zeiss LSM 710 NLO confocal microscope (Zeiss, White Plains, NY). Wavelength absorbance‐emission values were DAPI (410‐449), Adam17 (484‐552), and Aif (599‐670). Using an oil immersion objective, z‐stacks of the mPFC were obtained at 63× magnification (Objective i Plan‐Apochromat 63x/1.4 Oil DIC M27; field of view = 25 mm). Additional images were captured using an Andor BC43 Spinning Disk Confocal system (Andor Technology, Belfast) with an oil immersion objective (Plan Apo 60×, NA 1.4; Nikon). Excitation was achieved using 405 nm, 488 nm, 561 nm, and 640 nm lasers in sequence, and emission light was detected by an Andor sCMOS camera (4.2MP; 6.5 µm pixel size).

All the slides were scanned with the Zeiss LSM 710 NLO confocal microscope (Zeiss, White Plains, NY). Wavelength absorbance-emission values were as follows: DAPI (410–449); ADAM17 (484–552); Drd1 (552–601); Aif (599–670). Using an oil immersion objective, z-stacks of the mPFC were obtained at 63x magnification. Additional images were captured using an Andor BC43 Spinning Disk Confocal system (Andor Technology, Belfast) using an oil immersion objective (Plan Apo 60x, NA 1.4; Nikon). For excitation, 405 nm, 488 nm, 561 nm, and 640nm lasers were used in sequence. Emission light was detected by an Andor sCMOS camera (4.2MP; 6.5 um pixel size).