After lysis and denaturation of HK2 cells or kidney tissues from each group using the radioimmunoprecipitation assay (RIPA) buffer, proteins (50 μg) were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto nitrocellulose (NC) membranes. After blocking with 1x casein for 1 h to prevent non-specific binding, NC membranes were incubated with the following primary antibodies overnight at 4 °C: (a) rabbit monoclonal E-cadherin antibody (BD Bioscience, USA) diluted 1:100, (b) rabbit monoclonal anti-α-SMA antibody (Abcam, UK) diluted 1:300, (c) mouse monoclonal anti-CTGF antibody (Abcam) diluted 1:200, (d) rabbit polyclonal anti-fibronectin antibody (Proteintech, USA) diluted 1:500, (e) rabbit polyclonal Col3A1 antibody (Proteintech) diluted 1:500, and (f) mouse monoclonal β-actin antibody (Beyotime, China) diluted 1:10,000. The membranes were washed with TBST (Tris-buffered saline with Tween-20, 20 mM of Tris, 140 mM of NaCl, and 0.1% Tween-20) and then probed with 1:1000 diluted secondary antibodies at room temperature (25 °C) for 2 h. Enhanced chemiluminescence (ECL) western blotting kit (APPLYGEN, China) was used to detect the target bands, and β-actin was used as an internal reference to calculate the relative expression levels of proteins in each experimental group.
Rabbit monoclonal e cadherin antibody
Manufactured by BD
Sourced in United States
The Rabbit monoclonal E-cadherin antibody is a laboratory reagent used for the detection of the E-cadherin protein in biological samples. E-cadherin is a cell-cell adhesion molecule that plays a critical role in maintaining the integrity of epithelial tissues. This antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of E-cadherin in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti ctgf antibody, by Abcam (3 mentions)
Col3a1 antibody, by Proteintech (3 mentions)
Rabbit monoclonal anti α sma antibody, by Abcam (3 mentions)
β actin mouse monoclonal antibody, by Beyotime (3 mentions)
Ecl western blotting kit, by Applygen (3 mentions)
Anti bronectin antibody, by Proteintech (2 mentions)
Anti fibronectin antibody, by Proteintech (1 mentions)
3 protocols using rabbit monoclonal e cadherin antibody
1
Western Blot Analysis of Fibrosis Markers
2
Western Blot Analysis of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After lysis and denaturation of HK2 cells or kidney tissues from each group using the radioimmunoprecipitation assay (RIPA) buffer, proteins (50 μg) were separated by 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto nitrocellulose (NC) membranes. After blocking with 1x casein for 1 h to prevent non-speci c binding, NC membranes were incubated with the following primary antibodies overnight at 4°C : (a) rabbit monoclonal E-cadherin antibody (BD Bioscience, USA) diluted 1:100; (b) rabbit monoclonal anti-α-SMA antibody (Abcam, UK) diluted 1:300; (c) mouse monoclonal anti-CTGF antibody (Abcam) diluted 1:200; (d) rabbit polyclonal anti-bronectin antibody (Proteintech, USA) diluted 1:500; (e) rabbit polyclonal Col3A1 antibody (Proteintech) diluted 1:500; (f) mouse monoclonal β-actin antibody (Beyotime, China) diluted 1:10000. The membranes were washed with TBST (Tris-buffered saline with Tween-20, 20 mM of Tris, 140 mM of NaCl, and 0.1% Tween-20) and then probed with 1:1000 diluted secondary antibodies at room temperature (25ºC) for 2 h. Enhanced chemiluminescence (ECL) western blotting kit (APPLYGEN, China) was used to detect the target bands, and β-actin was used as an internal reference to calculate the relative expression levels of proteins in each experimental group.
3
Western Blot Analysis of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After lysis and denaturation of HK2 cells or kidney tissues from each group using the radioimmunoprecipitation assay (RIPA) buffer, proteins (50 μg) were separated by 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto nitrocellulose (NC) membranes. After blocking with 1x casein for 1 h to prevent non-speci c binding, NC membranes were incubated with the following primary antibodies overnight at 4°C : (a) rabbit monoclonal E-cadherin antibody (BD Bioscience, USA) diluted 1:100; (b) rabbit monoclonal anti-α-SMA antibody (Abcam, UK) diluted 1:300; (c) mouse monoclonal anti-CTGF antibody (Abcam) diluted 1:200; (d) rabbit polyclonal anti-bronectin antibody (Proteintech, USA) diluted 1:500; (e) rabbit polyclonal Col3A1 antibody (Proteintech) diluted 1:500; (f) mouse monoclonal β-actin antibody (Beyotime, China) diluted 1:10000. The membranes were washed with TBST (Tris-buffered saline with Tween-20, 20 mM of Tris, 140 mM of NaCl, and 0.1% Tween-20) and then probed with 1:1000 diluted secondary antibodies at room temperature (25ºC) for 2 h. Enhanced chemiluminescence (ECL) western blotting kit (APPLYGEN, China) was used to detect the target bands, and β-actin was used as an internal reference to calculate the relative expression levels of proteins in each experimental group.
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