E15103 34

All experimental treatments of animals were approved by the German Animal Welfare Authorities (approval no. G0016/11). All mice were maintained under conventional conditions and a 12:12 h light:dark cycle (lights on at 6:00 am) at a temperature of 22 ± 2 °C. Animals had ad libitum access to food and water. To perform fine mapping of the obesity QTL (Arends et al. 2016) , generation 28 was fed with a rodent high-fat diet (HFD) containing 19.5 MJ/kg of metabolizable energy, 45% from fat, 24% from protein and 31% from carbohydrates (E15103-34, ssniff EF R/M, Ssniff Spezialdiäten GmbH, Soest / Germany). All other generations used in this study were fed a standard breeding diet (V1534-000, ssniff EF R/M; Ssniff Spezialdiäten GmbH, Soest, Germany).

Mice were maintained under conventional conditions and a 12:12 h light:dark cycle (lights on at 6:00 a.m.) at a temperature of 22 ± 2°C. Animals had ad libitum access to food and water. They were fed with a rodent high-fat diet (HFD) containing 19.5 MJ/kg of metabolizable energy, 45% from fat, 24% from protein and 31% from carbohydrates (E15103-34, ssniff EF R/M, Ssniff Spezialdiäten GmbH, Soest / Germany). All experimental treatments of animals were approved by the German Animal Welfare Authorities (approval no. G0065/14).