DFX, penicillin, streptomycin, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium
bromide (MTT), Span 60, Tween 60, and ergosterol were obtained from
Sigma-Aldrich (Steinheim am Albuch, Germany and St Louis, MO, USA).
PBS, trypan blue,l -glutamine, 0.25% trypsin–ethylenediaminetetraacetic
acid solution, and dimethyl sulfoxide (DMSO) were procured from INOCLON
(Tehran, Iran). Fetal bovine serum (FBS) and Dulbecco’s modified
Eagle’s medium (DMEM) were purchased from Gibco (Grand Island,
NY, USA). Cholesterol hemisuccinate (CHEMS) was obtained from Avanti
Polar, USA. Caspase-3 assay kit was procured from R&D (R&D
Systems Co., Grodig, Germany). Michigan Cancer Foundation-7 (MCF-7)
breast cancer cells, Hela cervical cancer cells, and normal human
umbilical vein endothelial cells (HUVECs) were obtained from the cell
bank of Pasteur Institute of Iran (Tehran, Iran) and grown in DMEM
culture medium supplemented with the antibiotic mixture (50 U/mL of
penicillin and 50 μg/mL of streptomycin) and 10% heat-inactivated
FBS. Cells were incubated at 37 °C in a humidified atmosphere
with 5% CO2.
bromide (MTT), Span 60, Tween 60, and ergosterol were obtained from
Sigma-Aldrich (Steinheim am Albuch, Germany and St Louis, MO, USA).
PBS, trypan blue,
acid solution, and dimethyl sulfoxide (DMSO) were procured from INOCLON
(Tehran, Iran). Fetal bovine serum (FBS) and Dulbecco’s modified
Eagle’s medium (DMEM) were purchased from Gibco (Grand Island,
NY, USA). Cholesterol hemisuccinate (CHEMS) was obtained from Avanti
Polar, USA. Caspase-3 assay kit was procured from R&D (R&D
Systems Co., Grodig, Germany). Michigan Cancer Foundation-7 (MCF-7)
breast cancer cells, Hela cervical cancer cells, and normal human
umbilical vein endothelial cells (HUVECs) were obtained from the cell
bank of Pasteur Institute of Iran (Tehran, Iran) and grown in DMEM
culture medium supplemented with the antibiotic mixture (50 U/mL of
penicillin and 50 μg/mL of streptomycin) and 10% heat-inactivated
FBS. Cells were incubated at 37 °C in a humidified atmosphere
with 5% CO2.