Penicillin streptomycin

Manufactured by Keygen Biotech

Sourced in China, United States, Israel, Germany

Penicillin/streptomycin is a broad-spectrum antibiotic solution. It is commonly used to prevent bacterial contamination in cell culture applications.

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Close spelling variants of this product

Penicillin (60 citations) Streptomycin (58 citations) Penicillin–streptomycin solution (11 citations) Penicillin and streptomycin (8 citations) Penicillin-streptomycin mixture (2 citations)

58 protocols using penicillin streptomycin

Modulating miR-382 and PTEN/Akt Signaling in Liver Cells

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Mouse NCTC1469 normal liver cells were maintained in Dulbecco's Modified Eagle's Medium (Hyclone, USA) supplemented with 10% fetal bovine serum (Hyclone, USA) and 1% penicillin-streptomycin (Keygen, China) at 37°C in 5% CO₂ environment. Human HL7702 normal liver cells were maintained in RPMI-1640 medium (Hyclone, USA) supplemented with 20% fetal bovine serum (Hyclone, USA) and 1% penicillin-streptomycin (Keygen, China) at 37°C in 5% CO₂ environment.
For overexpression or suppression of miR-382, cells were transfected with miR-382 mimics (50 nM, RiboBio, China), miR-382 inhibitor (100 nM, Ribobio, China), or their negative controls for 48 hrs, respectively. To further investigate the role of PTEN and Akt in miR-382-associated hepatocyte proliferation and cell growth, cells were transfected with siRNA-PTEN (sequence-01 or 02, 75 nM, Ribobio, China) or siRNA-negative control for 48 hrs, or treated with Akt activator SC 79 (4 μg/mL, No. 4635, Tocris, UK) or Akt inhibitor LY 294002 (25 μM, No. 1130, Tocris, UK) for 24 hrs, respectively.

Bei Y., Song Y., Wang F., Dimitrova-Shumkovska J., Xiang Y., Zhao Y., Liu J., Xiao J, & Yang C. (2015). miR-382 targeting PTEN-Akt axis promotes liver regeneration. Oncotarget, 7(2), 1584-1597.

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Cell Culture Conditions for Cell Lines

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The CCRF-CEM and Ramos cells were cultured in 1640 medium with the addition of 10% FBS (fetal bovine serum, Invitrogen, Carlsbad, CA, USA) and 0.5 mg mL⁻¹ penicillin–streptomycin (KeyGEN Biotech, Nanjing, China) in a 5% CO₂ environment at 37 °C, A549 and HEK293 cells were cultured in DMEM medium with the addition of 10% FBS (fetal bovine serum, Invitrogen, Carlsbad, CA, USA) and 0.5 mg mL⁻¹ penicillin–streptomycin (KeyGEN Biotech, Nanjing, China) in a 5% CO₂ environment at 37 °C.

Tan Y., Xiong M., Liu Q., Yin Y., Yin X., Liao S., Wang Y., Hu L, & Zhang X.B. (2022). Precisely controlling the cellular internalization of DNA-decorated semiconductor polymer nanoparticles for drug delivery. RSC Advances, 12(48), 31173-31179.

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Neonatal Mouse Ovary Cultivation

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Ovaries were retrieved from neonatal mice and cultured in serum-free Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F12 nutrient mixture (Gibco, USA) with 100 IU/mL penicillin-streptomycin (KGY0023, Keygen Biotech, China). The HEK293T cell line was purchased from Keygen Biotech (KG405) and cultured in DMEM (Gibco) containing 10% fetal bovine serum (SE100-B, VISTECH, New Zealand) and 100 IU/mL penicillin-streptomycin (Keygen Biotech). The ovaries and cells were maintained at 37° C with 5% CO₂ and saturated humidity in a humidified incubator.

Zhao P., Song Z., Wang Y., Cai H., Du X., Li C., Lv J., Liu X., Guo M, & Chen Z. (2020). The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles. Aging (Albany NY), 13(1), 1096-1119.

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RSC96 Schwann Cell Culture Protocol

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Cells of the rat Schwann cell line RSC96 (BNCC341121; Beijing Pujing Kangli Technology Co., Ltd.) were inoculated into 7 mL of Dulbecco's modified Eagle medium (DMEM, containing 4.5 g/L D-glucose, L-glutamine, and 110 mg/L sodium pyruvate; Gibco) supplemented with 10% fetal bovine serum (Gibco, 10270–106) and 5% penicillin-streptomycin (Keygentec, KGY002) in T25 culture flasks. Next, the flasks were shaken vigorously to evenly disperse the cells and then incubated at 37°C with 5% CO₂. The cell culture was then passaged after reaching 70% confluence when observed under the microscope.

Wei X., Zheng Y., Ai Y, & Li B. (2022). Regulatory Effects of Astragaloside IV on Hyperglycemia-Induced Mitophagy in Schwann Cells. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 7864308.

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Isolation and Culture of Human Adipose-Derived Cells

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Samples of human adipose tissue were obtained from three healthy female donors undergoing thigh liposuction. Informed consent was obtained from these donors and the study protocol was approved by an institutional review board. The aspirated fat was washed with PBS and then digested with 0.1% collagenase type I (Gibco, Life Technologies, CA, USA). The digested tissue was centrifuged to separate the stromal cell fraction from adipocytes and connective tissues. The cells were resuspended with complete Dulbecco's modified Eagle medium (DMEM) (Gibco) containing 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Keygentec, Nanjing, China). The cells were passed through a 200-μm mesh and then centrifuged. The isolated cells were resuspended in complete DMEM, placed into 25-cm 2 flasks, and cultured at 37°C in a humidified atmosphere with 5% CO 2 . The medium was replaced every 3 days. Cells were passaged at a ratio of 1:2 per week. Only cells that were passaged 4 times were used in this study.

Cui Z., Zhou H., He C., Wang W., Yang Y, & Tan Q. (2015). Upregulation of Bcl-2 enhances secretion of growth factors by adipose-derived stem cells deprived of oxygen and glucose. Bioscience trends, 9(2).

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Assessing Radiation Resistance in HGC-27 Cells

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Cell lines and cell culture. The HGC-27 cell line was obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained in a T-25 flask with RPMI-1640 (Gibco, Grand Island, NY, USA), supplemented with 1% penicillin streptomycin (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) and 10% fetal bovine serum (FBS; Gibco). The cells were incubated at 37˚C, in a 5% CO 2 humidified incubator. Colony formation assay. Cells (2x10 5 ) were distributed in 6-well plates and allowed to adhere for 24 h. Then, the cells were treated with vehicle control or [6] -gingerol (300 µM) for 24 h, followed by exposure to different doses of IR. Cells were harvested, counted and 500 cells of each treatment were seeded into a 60-mm culture dish with fresh complete culture medium. Following 10-14 days of incubation, the colonies were stained with crystal violet staining solution (Beyotime Institute of Biotechnology, Shanghai, China). The plates were pictured using a digital camera, and the surviving colonies (colonies containing more than 50 cells under a microscope in x100 magnification) were counted by Adobe Photoshop CS6 (Adobe, San Jose, CA, USA). Cell survival curves were fitted with the linear-quadratic model using GraphPad Prism 5 software (GraphPad Software, Inc., La jolla, CA, USA).

Luo Y., Chen X., Luo L., Zhang Q., Gao C., Zhuang X., Yuan S, & Qiao T. (2018). [6]-Gingerol enhances the radiosensitivity of gastric cancer via G2/M phase arrest and apoptosis induction. Oncology reports, 39(5).

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Immunostaining of Microtubules in MRC-5 Cells

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Human embryonic
lung fibroblast
(MRC-5) cells were purchased from China Type Culture Collection. Cells
were kept under standard cell culture conditions (5% CO₂, 37 °C). The culture media (Dulbecco’s modified Eagle’s
medium, DMEM) were supplemented with 10% fetal bovine serum (GIBCO)
and 1% penicillin–streptomycin (Nanjing KeyGen Biotech. Co.,
Ltd.). For fluorescence imaging, MRC-5 cells were seeded into cell
culture dishes and incubated for 24 h before use. Microtubules were
immunostained using the following procedure: washing in PBS; fixation
in a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in PBS
for 20 min; 3× washing with PBS; reduction with 1 mg/mL of NaBH₄ for 7 min; 3× washing with PBS; permeabilization with
0.2% (v/v) Triton X-100 in PBS for 30 min; 3× washing with PBS;
blocking with 3% (w/v) bovine serum albumin (BSA) for 90 min and staining
overnight with the mouse antibody against β-tubulin; 3×
washing with PBS; post-fixation in a mixture of 4% paraformaldehyde
and 0.1% glutaraldehyde in PBS for 10 min.

Lu J., Zong S., Wang Z., Chen C., Zhang Y., Wang H, & Cui Y. (2021). Dual-Labeled Graphene Quantum Dot-Based Förster Resonance Energy Transfer Nanoprobes for Single-Molecule Localization Microscopy. ACS Omega, 6(13), 8808-8815.

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C2C12 Myoblast Differentiation and Atrophy

Cited 1 time

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C2C12 cells (mouse skeletal myoblasts) were obtained from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Corning, NYC, USA, 10-013-CV) with 10% Fetal bovine serum (Biological Industries, Beit HaEmek, Israel) and 1% Penicillin-Streptomycin (KeyGEN, Nanjing, China, KGY0023) at 37 ℃ supplemented with 5% CO₂. To differentiate into myotubes, C2C12 myoblasts cells were cultured in a differentiation medium (DMEM containing 2% horse serum and 1% penicillin and streptomycin) and the entire differentiation process lasted about 4 days. EV-depleted Fetal bovine serum (FBS) was obtained after overnight centrifugation at 100,000 g at 4 °C (Beckman Coulter, Avanti JXN-30). For screening the optimum concentration, EVs were added to the medium for 24 h at different doses. For determining the function of EVs in muscle atrophy in vitro, EVs were added to the medium at a dose of 1 × 10¹⁰ particles/mL for 24 h, then muscle atrophy was induced. Dexamethasone (Dex, Sigma, MO, USA, D4902), TNF-α (PeproTech, NJ, USA, 315-01 A), and Ang II (Sigma, MO, USA, A9525) were used to induce muscle atrophy at the cellular level according to previously reported methods [4 (link)].

Chen R., Yuan W., Zheng Y., Zhu X., Jin B., Yang T., Yan Y., Xu W., Chen H., Gao J., Li G., Gokulnath P., Vulugundam G., Li J, & Xiao J. (2022). Delivery of engineered extracellular vesicles with miR-29b editing system for muscle atrophy therapy. Journal of Nanobiotechnology, 20, 304.

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Culturing HepG2 and SH-SY5Y Cell Lines

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HepG2 cells were cultured in DMEM supplemented with 10% FBS and 100 U/mL of penicillin-streptomycin (Thermo, Waltham, MA, USA) at 37 °C in a humidified atmosphere containing 5% CO₂.
SH-SY5Y cells were cultured in Dulbecco’s modified Eagle medium (DMEM) with F12 (KeyGEN BioTECH, Nanjing, China), supplemented with 10% (v/v) fetal bovine serum (FBS) (Sen Bei Jia) and antibiotics (penicillin streptomycin) (KeyGEN BioTECH, Nanjing, China). Cells were kept at 37 °C and 5% CO₂ in a humidified atmosphere in a cell culture incubator and passaged twice a week.

Wu X., Ze X., Qin S., Zhang B., Li X., Gong Q., Zhang H., Zhu Z, & Xu J. (2024). Design, Synthesis, and Biological Evaluation of Novel Tetrahydroacridin Hybrids with Sulfur-Inserted Linkers as Potential Multitarget Agents for Alzheimer’s Disease. Molecules, 29(8), 1782.

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Isolating Breast Cancer-Derived Conditioned Medium

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Human umbilical vein endothelial cells (HUVECs) and MDA-MB-231 cells were supplied by Capital Medical University (Beijing, China). MDA-MB-231 cells (base-like) were classified as ER-, PR-, and HER2- [34] [35] [36] [37] . MDA-MB-231 cells were cultured in Dulbecco's modified Eagle medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin/streptomycin (KeyGen, Nanjing, China) and maintained in a 5% CO 2 atmosphere at a constant temperature of 37°C
. Jiangsu Hengrui Medicine Co., Ltd. (Jiangsu, China) supplied apatinib free of cost, whereas DHA was ordered from Sigma (St. Louis, MO, USA; D2534). Dimethyl sulfoxide was used to dissolve apatinib, while 100% ethyl alcohol (<0.1%) was utilized to prepare a solution of DHA. Both solutions were freshly prepared for each test.
Preparation of MDA-MB-231-conditioned medium (CM)
Isolation of MDA-MB-231-CM was performed as described previously [38, 39] . Cultures of MDA-MB -231 cells were prepared in 100-mm 2 plates until they reached 70% confluence, and then they were cultured in 0-1% FBS for 24-48 h. The medium was extracted using 0.22-μm filter film and stored at -80°C.

Ma Y., Yu J., Li Q., Su Q, & Cao B. (2020). Addition of docosahexaenoic acid synergistically enhances the efficacy of apatinib for triple-negative breast cancer therapy. Bioscience, biotechnology, and biochemistry, 84(4).

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