The oligodeoxynucleotides were synthesized and purified by Shanghai Sangon Biological Engineering Technology & Services Co, Ltd (Shanghai, China). ATP, ATPγS (adenosine 5′-(γ-thio) triphosphate tetralithium), NADH (β-nicotinamide adenine dinucleotide, reduced disodium salt), and pyruvate kinase/lactic dehydrogenase were supplied by Sigma-Aldrich (St Louis, MO, USA). Phosphoenolpyruvate was purchased from Ruibio-bio (Ingelheim, Germany). Wild type RecA protein (E. coli) was purchased from New England Biolabs (Ipswich, MA, USA) or expressed and purified in our lab. All RecA mutants were expressed and purified in our own lab. Supernuclease was obtained from Sino Biological Inc. (Beijing, China). Ultrapure water of 18.2 MΩ cm was obtained from a Purelab Ultra Bioscience system (ELGA, UK). All other reagents and solvents were analytical grade and supplied by Beijing Chemical Reagents (Beijing, China).
Oligodeoxynucleotides
Manufactured by Sangon
Sourced in China
Oligodeoxynucleotides are short, synthetic DNA sequences that are used as research tools in various scientific applications. They are typically composed of 15-30 nucleotides and are designed to target specific DNA or RNA sequences. Oligodeoxynucleotides can be used for a variety of purposes, including gene expression analysis, antisense technology, and the creation of DNA-based sensors and probes.
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2 protocols using oligodeoxynucleotides
1
Purification and Characterization of RecA Proteins
2
Oligodeoxynucleotide Characterization and Preparation
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Oligodeoxynucleotides (see Supplementary Table S1 in Supporting Information) were synthesized by Sangon Biotechnology (Shanghai, China), and used directly without further purification. All were dissolved in 10 mM Tris–EDTA buffer (pH 8.0) as stock solutions and determined by ultraviolet (UV) spectrometry using the extinction coefficient (ϵ260 nm, M−1▪cm−1). The stock solutions were stored in a refrigerator at 4°C before use. Thioflavin T was purchased from Sigma-Aldrich (USA). Magnesium acetate (MgAc2), silver nitrate (AgNO3) and mercury acetate (HgAc2) were obtained from Energy Chemical. All chemical reagents were of reagent grade and used without further purification.
Before all experiments, all DNAs were diluted to 40 mM TA (Tris–Ac, pH 8.0) buffer to desired concentrations and heated at 90°C for 10 min and cooled down to room temperature gradually.
Before all experiments, all DNAs were diluted to 40 mM TA (Tris–Ac, pH 8.0) buffer to desired concentrations and heated at 90°C for 10 min and cooled down to room temperature gradually.
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