Mag mk virus rna extraction kit

Tris (2-carboxyethyl) phosphine hydrochloride (TCEP, 98.0%) and 6-Mercapto-1-hexanol (MCH, 98.0%) were purchased from Sigma-Aldrich. The fluorescence ERA (No. KS103) and RT-ERA (No. ZK009) reaction kits were purchased from GenDx Biotech (Suzhou, China). The Mag-MK Virus RNA Extraction Kit (NO. B518767), magnetic separation device (NO. B518800), One-Step RT-qPCR Probe Kit (NO. B639278), DEPC-treated water (DEPC, NO. B501005), 1 × TE buffer (NO. B548106), and RNase inhibitor (RNaseI) (NO. B600478) were purchased from Sangon Biotech (Shanghai, China). Nuclease-free water was purchased from TINGEN (RT-121, Beijing, China). SARS-CoV-2 RNA standard (No.H2101262/004) was purchased from SIMT (Shanghai, China). SARS-CoV-2 β-inactivated virus strain (ZK009) and γ-inactivated virus strain (ZK009), human SARS, and human influenza A viruses (H1N1, H3N2, H5N1, and H7N9 subtypes) were purchased from Bio come (Nanjing, China). Conventional primer, thiolated primer, fluorescence resonance energy transfer (FRET) probe, and plasmid DNA (orf1ab and N gene) associated with the SARS-CoV-2 virus were synthesized by Sangon Biotech (Shanghai, China).