The largest database of trusted experimental protocols

2 protocols using nigericin sodium salt

1

Disulfiram and Lactoferrin Therapeutic Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols
Disulfiram (DSF) was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). Lactoferrin (LF) was purchased from Nanjing Jingruijiuan Biotechnology Co., Ltd. (Nanjing, China). Soy lecithin and DSPE-mPEG2000 were purchased from AVT (Shanghai) Pharmaceutical Tech Co., Ltd. (Shanghai, China). Dulbecco’s Modified Eagle’s Medium (DMEM) cell culture medium and fetal bovine serum (FBS) were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Recombinant murine macrophage colony-stimulating factor (M-CSF) was purchased from PeproTech (Rocky Hill, NJ, USA). Mouse interleukin 1 beta (IL-1β) Elisa Kit was purchased from Shanghai Dakewe Biotech Co., Ltd. (Shanghai, China). Enhanced ATP Assay Kit was purchased from Beyotime Institute of Biotechnology (Haimen, China). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Nigericin sodium salt was purchased from MedChemexpress Co., Ltd. (Shanghai, China). Dextran sulfate sodium salt (DSS) was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. All other reagents (analytical grade) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) and were used without further purification.
+ Open protocol
+ Expand
2

NLRP3 Inflammasome Activation and Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for IL-1β, caspase-1, PP2A, and phospho-PP2A (Y307) were obtained from Abcam. NLRP3 and ASC antibodies were provided by R&D Systems and Adipogen respectively. Other antibodies used in this study include: β-actin (Proteintech Group), IBA-1 (GeneTex), TRPV1 (Alomone labs), Goat Anti-Rabbit IgG-HRP (CST), Goat Anti-Mouse IgG-HRP (CST), Goat Anti-Rat IgG-HRP (Proteintech Group), Dylight 488, Goat Anti-Rabbit IgG (Abbkine), Dylight 488, Goat Anti-Mouse IgG (Abbkine), Dylight 594, Goat Anti-Rabbit IgG (Abbkine), Dylight 594, and Goat Anti-Mouse IgG (Abbkine).
PAMPs used in this study were LPS (Escherichia coli 0111: B4, L2630, Sigma), ATP (MedChemExpress), and Nigericin sodium salt (MedChemExpress). Chemical inhibitors and reagents include: Capsazepine (CPZ, MedChemExpress), BAPTA-AM (MedChemExpress), Okadaic acid (OA, Beyotime Biotechnology), Z-VAD-FMK (MedChemExpress), Fluo-3 AM (Dojindo), and Pluronic F-127 (Sigma).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!