Supernatants from untreated and treated HCAEC were spun down (300 g, 5 min) and cell-free supernatants frozen at −80°C until usage. The assays were performed using commercial ELISA kits and were done in duplicates, according to the manufacturers‘ instructions. IL-6 and IL-8 ELISA kits were purchased from Invitrogen (Frederick, MD, USA), while GROa and MIF quantikine ELISA were from R&D Systems (Minneapolis, USA). Absorbance was measured at 450 nm with the microplate absorbance reader (Tecan, Groening, Austria). The concentrations of analytes were calculated from standard curves and multiplied by the dilution factor.
Mif quantikine elisa
Manufactured by R&D Systems
Sourced in United States
The MIF Quantikine ELISA is a quantitative sandwich enzyme immunoassay designed for the measurement of Macrophage Migration Inhibitory Factor (MIF) levels in cell culture supernates, serum, and plasma. It is a laboratory equipment product.
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2 protocols using mif quantikine elisa
1
Quantifying Inflammatory Cytokines in HCAEC
2
Quantification of CXCL12 and MIF Secretion
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CXCL12 and MIF secretion in CM was measured using the Human CXCL12/SDF-1 DuoSet ELISA and MIF Quantikine ELISA kits (R&D Systems) following the manufacturer’s protocols. The absorbance was measured at 450 nm and corrected at 540 nm on a microplate reader Infinite® 200 PRO (Tecan, Switzerland).
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