Duopafei

Docetaxel was purchased from Alfa Aesar (Shanghai, China). Poly(ethylene glycol)-b-poly(lactic acid) (mPEG-PLA, MW = 13,000 ∼ 100,000) and polylactic acid (PLA, MW = 5000) were purchased from Daigang Biomaterial Co., Ltd (Jinan, Shandong Province, China). Commercial Docetaxel injection (Duopafei^®) was acquired from Qilu Pharmaceutical Co., Ltd (Jinan, Shandong Province, China). Tween-80 and (2-Hydroxypropyl)-β-cyclodextrin were purchased from Sinopharm Chemical Reagent Co., Ltd (Beijing, China). Sodium cholate was purchased from Sigma-Aldrich (St. Louis, MO, USA). Polyvinyl alcohol was purchased from Kuraray Co., Ltd (Osaka, Osaka Prefecture, Japan). Cyclophosphamide for injection was purchased from Hengrui Pharmaceuticals Co., Ltd (Lianyungang, Jiangsu Province, China). All chemicals obtained were used without additional purification. Tumor-bearing nude mice (20 ± 2 g) and rats (200 ± 20 g) were purchased from Zhonghongboyuan Biotechnology Co., Ltd (Beijing, China). All animal experiments were conducted with the Guide for the Care and Use of Laboratory Animals of Beijing University of Chinese Medicine.