Brx 500

Manufactured by Bruker

The BRX-500 is a nuclear magnetic resonance (NMR) spectrometer manufactured by Bruker. It is designed to perform high-resolution NMR analysis of various chemical and biological samples. The BRX-500 provides a magnetic field strength of 500 MHz, allowing for precise identification and characterization of molecular structures.

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Lab products found in correlation

Avatar 330, by Thermo Fisher Scientific (1 mentions)

2 protocols using brx 500

Characterization of Synthesized Polymer MC

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The synthesized polymer MC was characterized by Fourier Transform infra-red spectroscope (FTIR) (Thermo Nicolet AVATAR 330, Waltham, MA, USA). IR spectra were acquired by the KBr disc method at 4 cm⁻¹ resolution at the wavenumber range of 400–4000 cm⁻¹ at room temperature (RT). Moreover, the ¹H NMR spectroscopy (Bruker BRX-500) was performed in deuterated DMSO to analyze the polymer.

Mukhtar M., Csaba N., Robla S., Varela-Calviño R., Nagy A., Burian K., Kókai D, & Ambrus R. (2022). Dry Powder Comprised of Isoniazid-Loaded Nanoparticles of Hyaluronic Acid in Conjugation with Mannose-Anchored Chitosan for Macrophage-Targeted Pulmonary Administration in Tuberculosis. Pharmaceutics, 14(8), 1543.

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Chitosan-Maleimide Conjugation Protocol

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In brief, maleimide-propionic acid (0.553 mg, 5:1 ratio CS:m-PA) in dimethyl sulfoxide (DMSO) was reacted with 5 mg of chitosan in the presence of 0.339 mg of NHS (2:1 ratio CS:NHS) and 0.188 mg of EDC (2:1 ratio CS:EDC) as crosslinkers and were kept under constant stirring for 24 h. Excess reagents were removed by dialysis using dialysis membrane (3.5 kDa) . The chemical conjunction between chitosan and maleimide-propionic acid was confirmed by H 1 -NMR spectrometry (Bruker BRX-500).

Robla S., Prasanna M., Varela-Calviño R., Grandjean C, & Csaba N. (2021). A chitosan-based nanosystem as pneumococcal vaccine delivery platform. Drug delivery and translational research, 11(2).

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