The variants in exon four in the APOE gene (GenBank: NM_000041 )—that is, c.388T > C and c.526C > T—were analyzed by polymerase chain reaction and Sanger sequencing in DNA extracted from peripheral leukocytes. Primers were designed using Primer3Plus (Bioin-formatics, Arlington, VA, USA) (34 (link)) and the polymerase chain reaction products were purified and Sanger sequenced using an ABI 3730xl DNA analyzer and an ABI BigDye terminator cycle-sequencing kit v3.1 (Thermo Fisher Scientific, Waltham, MA, USA). Sequences were analyzed with the DNA Sequencing Analysis software program (version 5.1; Applied Biosystems, Foster City, CA, USA) and the SeqScape software program (version 2.7; Thermo Fisher Scientific).
Dna sequencing analysis software program
Manufactured by Thermo Fisher Scientific
Sourced in United States
The DNA Sequencing Analysis software program from Thermo Fisher Scientific is a tool designed for the analysis of DNA sequence data. It provides a suite of tools to support the interpretation of DNA sequencing results.
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Lab products found in correlation
2 protocols using dna sequencing analysis software program
1
APOE Exon 4 Variant Analysis
2
Genotyping APOE Exon 4 Variants
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The variants in exon 4 in APOE (GenBank: NM_000041)—that is, c.388T>C and c.526C>T—were analyzed by polymerase chain reaction and Sanger sequencing in DNA extracted from peripheral leukocytes. Primers were designed using Primer3Plus (Bioinformatics, Arlington, VA, USA) [40 (link)] and the polymerase chain reaction products were purified and Sanger-sequenced using an ABI 3730xl DNA analyzer and ABI BigDye terminator cycle-sequencing kits v3.1 (Thermo Fisher Scientific, Waltham, MA, USA). Sequences were analyzed with the DNA Sequencing Analysis software program (version 5.1; Applied Biosystems, Foster City, CA, USA) and the SeqScape software program (version 2.7; Thermo Fisher Scientific).
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