Troponin 1 antibody

SalB and Rg1 were purchased from Shanghai Yousi Bio-Tech Co., Ltd (Shanghai, China). The structure of SalB (S1 Fig) or Rg1 (S2 Fig) was elucidated by ¹H and ¹³C NMR spectrum using Bruker AM-400 spectrometer. Purity of SalB was 99.84%, and Rg1 was 99.78% evaluated by high performance liquid chromatography (S3 Fig). Procarta cytokine profiling kit (Panomics, CA, USA), chloral hydrate (SCRC, Shanghai, China), 3,5-triphenyltetrazolium chloride (Sigma-Aldrich, USA), troponin-I antibody (abcam, Cambridge, United Kingdom), vimentin antibody (Calbiochem, Darmstadt, Germany), β-actin antibody (Cell-signaling, Boston, USA) and collagenase Type II (DingGuo, Shanghai, China) were commercially obtained. Mitochondrial membrane potential assay kit was purchased from Beyotime (Jiangsu, China). H9c2 cells were purchased from Cell bank of Chinese Academy of Sciences (Shanghai, China). All the culture reagents were products from Thermo Scientific (Beijing, China) unless specified otherwise.

AS-IV (batch number: 140913) was obtained from Weikeqi Biotechnology (Sichuan, China). Betloc tablets (batch number: 2010038) were purchased from AstraZeneca (Wuxi, China). Cell Counting Kit-8 (CCK-8, CAS Number: ZP328-3) was purchased from Zomanbio (Beijing, China). ATP Assay kit (CAS Number: S0026), mitochondrial membrane potential assay kit with JC-1 (CAS Number: C2006), and reactive oxygen species assay kit (CAS Number: S0033S) were purchased from Beyotime Biotechnology (Shanghai, China). RiboFECT transfection kit (CAS Number: C10511-1) was obtained from Ribo Biotechnology (Guangzhou, China). Mitochondrial isolation kit (Lot#019M4159V) was purchased from Sigma (St. Louis, USA). Troponin I antibody (CAS Number: ab209809), CD31 antibody (CAS Number: ab222783), α-smooth muscle actin (α-SMA) antibody (CAS Number: ab124964), BAX antibody (CAS Number: ab32503), Bcl2 antibody (CAS Number: ab196495), cytochrome C antibody (CAS Number: ab133504), Sirt3 antibody (CAS Number: ab246522), Opa1 antibody (CAS Number: ab42364), VDAC1 antibody (CAS Number: ab15895), and Drp1 antibody (CAS Number: ab184247) were obtained from Abcam (Cambridge, MA, USA).