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Mouse trap elisa

Manufactured by Immunodiagnostic Systems
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The Mouse TRAP ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure the concentration of TRAP (Tartrate-Resistant Acid Phosphatase) in mouse serum or plasma samples. TRAP is an enzyme that serves as a biomarker for bone resorption.

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Lab products found in correlation

Ratlaps eia, by Immunodiagnostic Systems (2 mentions) Rat mouse p1np eia, by Immunodiagnostic Systems (2 mentions) Mouse osteocalcin eia kit, by Biomedical Technologies (1 mentions) Ratlaps elisa, by Immunodiagnostic Systems (1 mentions) Ratlaps ctx 1 eia, by Immunodiagnostic Systems (1 mentions)

4 protocols using mouse trap elisa

1

Bone Remodeling Biomarker Quantification

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Blood was obtained from anesthetized mice by cardiac puncture at 6 weeks of age, and sera were stored in aliquots at -80°C. All ELISAs were performed as instructed by the manufacturer. C-terminal telopeptide (CTX) measurements were performed using the RatLaps ELISA (Immunodiagnostic Systems, Gaithersburg, MD; #AC-06F1). TRAcP 5b protein measurement was performed using Mouse TRAP ELISA (Immunodiagnostic Systems; #SB-TR103). Osteocalcin measurement was performed using the Mouse Osteocalcin EIA kit (Biomedical Technologies, Ward Hill, MA # BT-470). Assays were run in triplicate on between 3 and 9 individual animals per genotype.
Witwicka H., Hwang S.Y., Reyes-Gutierrez P., Jia H., Odgren P.E., Donahue L.R., Birnbaum M.J, & Odgren P.R. (2015). Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse Model, Rescue of Cell Fusion, and Transmembrane Topology. PLoS ONE, 10(6), e0128275.
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2

Serum Collection and Bone Marker Analysis

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Blood was collected by retro-orbital bleeding and allowed to clot at room temperature for 1 hour, after which the blood was centrifuged for 10 minutes at 1000g and the serum collected and stored in aliquots at –80°C. Kits for measuring TRAP5b (MouseTRAP ELISA), CTX-1 (RatLaps EIA), and P1NP (Rat/Mouse P1NP EIA) were purchased from Immunodiagnostic Systems, Inc., and used according to the manufacturer’s instructions.
Fu Q., Bustamante-Gomez N.C., Reyes-Pardo H., Gubrij I., Escalona-Vargas D., Thostenson J.D., Palmieri M., Goellner J.J., Nookaew I., Barnes C.L., Stambough J.B., Ambrogini E, & O’Brien C.A. (2023). Reduced osteoprotegerin expression by osteocytes may contribute to rebound resorption after denosumab discontinuation. JCI Insight, 8(18), e167790.
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3

Bone Metabolism Serum Markers Assay

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Serum levels of bone metabolism were assessed by colorimetric ELISA assays. Procollagen type 1 N-terminal pro-peptide (P1NP), a marker of bone formation, was measured using Rat/Mouse P1NP enzyme immunoassay (EIA) (Immunodiagnostic Systems, Fountain Hills, AZ, USA), carboxy-terminal collagen crosslinks, a marker of bone resorption, was measured using RatLaps EIA (Immunodiagnostic Systems), and TRAP activity, a marker of osteoclast number, was measured with Mouse TRAP ELISA (Immunodiagnostic Systems).
Weivoda M.M., Ruan M., Pederson L., Hachfeld C., Davey R.A., Zajac J.D., Westendorf J.J., Khosla S, & Oursler M.J. (2015). Osteoclast TGF-β Receptor Signaling Induces Wnt1 Secretion and Couples Bone Resorption to Bone Formation. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 31(1), 76-85.
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4

Bone Turnover Markers Measurement

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Circulating levels of procollagen type 1 amino-terminal propeptide (P1NP; bone formation marker), tartrate-resistant acid phosphatase 5b (TRAcP5b; bone resorption marker) and collagen type 1 cross-linked C-telopeptide (CTX; bone resorption marker) were measured with colorimetric assays (Rat/Mouse P1NP EIA #AC-33F1, Immunodiagnostic Systems, Fountain Hills AZ; MouseTRAP ELISA #SB-TR103, Immunodiagnostic Systems, Fountain Hills AZ; RatLaps (CTX-I) EIA #AC-06F1, Immunodiagnostic Systems, Fountain Hills AZ). All samples were tested in duplicate within each assay.
McGee-Lawrence M.E., Pierce J.L., Yu K., Culpepper N.R., Bradley E.W, & Westendorf J.J. (2017). Loss of Hdac3 in osteoprogenitors increases bone expression of osteoprotegerin, improving systemic insulin sensitivity. Journal of cellular physiology, 233(4), 2671-2680.
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