Multiplex immunoassay

Manufactured by Eve Technologies

Sourced in Canada

The Multiplex immunoassay is a laboratory equipment used to simultaneously detect and quantify multiple analytes in a single sample. It employs a combination of antibodies and fluorescent dyes to identify and measure the presence of specific proteins, cytokines, or other biomolecules in a sample. The core function of this equipment is to provide efficient and high-throughput analysis of complex biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Infinite 200 pro microplate reader, by Tecan (1 mentions) Solid phase sandwich elisa, by R&D Systems (1 mentions) Flat bottomed white walled plate, by Corning (1 mentions) Biolux gaussia luciferase reagent, by New England Biolabs (1 mentions) Fireplex immunoassay, by Abcam (1 mentions) Pierce bicinchoninic acid protein assay kit, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions)

Close spelling variants of this product

Bead-based multiplex immunoassays (4 citations)

3 protocols using multiplex immunoassay

Luminescence and ELISA Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

For luminescence assays, media was harvested 24 hours post-transfection. To detect luminescence from Gaussia luciferase, 20uL of tissue culture medium was transferred to a flat-bottomed white-walled plate (Corning). 25uL of BioLux Gaussia Luciferase reagent including stabilizer (New England Biolabs) was added to each sample and luminescence was measured on an Infinite 200Pro Microplate Reader (Tecan) after 45 seconds. Human erythropoietin was detected by solid phase sandwich ELISA (R&D Systems) essentially according to the manufacturer’s instructions. Cytokines in Figures 1, 3 and 5 were detected by Fireplex immunoassay (Abcam). Cytokines in Figures 2 and 6 were detected by individual or multiplex immunoassay (Eve Technologies). For all multi-day GLuc and hEpo data, expression is presented relative to the first day of expression for each condition.

Wesselhoeft R.A., Kowalski P.S., Parker-Hale F.C., Huang Y., Bisaria N, & Anderson D.G. (2019). RNA circularization diminishes immunogenicity and can extend translation duration in vivo. Molecular cell, 74(3), 508-520.e4.

+ Open protocol

+ Expand

Characterization of Ileal Mucosal Disaccharidase and IgA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ileal mucosal scrapings (0.5 g) were added to 4.5 mL of PBS containing a protease inhibitor cocktail (Sigma Aldrich, St. Louis, MO) and triton (0.1%). The resulting solution was homogenized and centrifuged at 10,000 × g for 15 min at 4 °C and the supernatant was stored in aliquots. Total protein concentration of hydrolyzed mucosa was quantified using a Pierce bicinchoninic acid Protein Assay kit (Thermo Scientific, Woltham, MA). The intra-assay coefficient of variation was 6.4%. Disaccharidase-specific activity was determined as previously described by Dahlqvist (1964) (link) using lactose, maltose, and sucrose as substrates. The intra- and interassay coefficients of variation was 7.3 and 14.7%. Enzyme activity was expressed as μmol hydrolyzed substrate × min-1×g tissue protein-1. Concentration of secretory IgA was obtained using a porcine-specific ELISA kit following manufacturer’s instructions (Bethyl Laboratories, Inc., Montegomery, TX). The intra-assay coefficient of variation was 3.8%. Homogenized mucosa and plasma subsamples were analyzed for cytokines using a Multiplex Immunoassay (Eve Technologies, Calgary, AB, Canada).

Becker S.L., Li Q., Burrough E.R., Kenne D., Sahin O., Gould S.A, & Patience J.F. (2020). Effects of an F18 enterotoxigenic Escherichia coli challenge on growth performance, immunological status, and gastrointestinal structure of weaned pigs and the potential protective effect of direct-fed microbial blends. Journal of Animal Science, 98(5), skaa113.

+ Open protocol

+ Expand

Multiplex Cytokine Quantification in BALF

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cytokine protein levels in the bronchoalveolar lavage fluids (BALF) were determined by multiplex immunoassay (Eve Technologies, Calgary, AB, Canada). All samples were twofold diluted in 1% triton X-100 (final) for decontamination.

Wu W., Tian L., Zhang W., Booth J.L., Ritchey J.W., Wu S., Xu C., Brown B.R, & Metcalf J.P. (2022). Early IFN-β administration protects cigarette smoke exposed mice against lethal influenza virus infection without increasing lung inflammation. Scientific Reports, 12, 4080.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!