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2 protocols using n dimethylformamide dmf

1

Optimization of Cellular Imaging Reagents

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O-phenylenediamine (oPD), thiourea, glyceryl trioleate and Erastin were obtained from Aladdin Reagent Co. n-Octano and oleic acid were obtained from macklin, ShangHai. Dioxane, dichloromethane (DCM), acetone, N, N-dimethylformamide (DMF) and dimethyl sulfoxide (DMSO), ethanol and methanol were purchased from Kelong Chemical Reagent Factory Chengdu, Quinine. Sulfate was obtained from Bide Pharmatech Co., Ltd. BODIPY493/503 was obtained from Thermo Fisher. RedDotTM1 came from Biotium. Mito Tracker Red, Lyso-Tracker Red and ER-Tracker Red were purchased from Beyotime Institute of Biotechnology, Shanghai, China. Dulbecco's Modified Eagle Medium (DMEM), alpha-Modified Eagle Medium and Hank’s balanced salt solution (HBSS) media came from Hyclone (USA). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoli-um bromide) was purchased from Beijing Solarbio Science and Technology, China. All chemicals were analytical grade and used without any further purification.
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2

Synthesis of Silane-Functionalized Nanoparticles

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Methylbenzene(C7H8), 3–(trimethoxysilyl)−1–propanamine (KH−540), ethanol (C2H5OH), formic acid (HCOOH), formaldehyde (HCHO), hydrochloric acid (HCl), N, N–dimethylformamide (DMF), and sodium hydroxide (NaOH) were purchased from Chengdu Kelong Chemical Reagent Factory (Sichuan, China). Nano–SiO2 (10–20 nm) was obtained from Aladdin Chemistry Co. (Shanghai, China). All chemical reagents were analytical–grade. CO2 (g) and N2 (g) were purchased from Jingli Gas Company (Chengdu, China). Water was double deionized with a Millipore Milli Q system to produce the 18 MM deionized water.
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