7890b gas chromatography

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 7890B Gas Chromatograph is a versatile laboratory instrument designed for the analysis of complex mixtures of volatile organic compounds. It features a high-performance oven, sensitive detectors, and advanced software control for accurate and reproducible separation and identification of sample components.

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Lab products found in correlation

Td100 xr thermal desorption autosampler, by Markes International (1 mentions) 5977a mass spectrometer, by Agilent Technologies (1 mentions) 5977 mass spectrometer, by Agilent Technologies (1 mentions) Masshunter quantitative analysis software, by Agilent Technologies (1 mentions) Hp 5ms capillary column, by Agilent Technologies (1 mentions) Glyceryl triheptadecanoate, by Merck Group (1 mentions) Db 23 column, by Agilent Technologies (1 mentions)

7 protocols using 7890b gas chromatography

Thermal Desorption Gas Chromatography-TOF MS

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Samples were dry purged to remove excess water and desorbed using a TD100-xr thermal desorption autosampler (Markes International) and transferred onto a Quadrex 007-624 column (30 m × 0.32 mm × 3.00 µm) using splitless injection. Chromatographic separation was achieved via a programmed method (40–250 °C in 84.5 min at 3.0 mL/min) on a 7890B gas chromatography (GC) oven (Agilent Technologies, Santa Clara, CA, USA) and mass spectral data acquired using an electron impact ionization time-of-flight (TOF) BenchTOF high-definition mass spectrometer (MS) (Markes International). Each sample consisted of two sorbent tubes, both of which were desorbed into the Thermal Desorber cold trap for a single analysis. A cleaning method was run between each sample to prevent carry-over.
A quality control (QC) sample (sorbent tube spiked with a known mixture of chemicals) was run between every four subject breath samples to monitor the stability of instrumentation. A blank tube was run every four samples and after every quality control sample to monitor background. A set of four samples, quality control samples, and blank tubes are denoted as an “analytical sequence”.

Azim A., Rezwan F.I., Barber C., Harvey M., Kurukulaaratchy R.J., Holloway J.W, & Howarth P.H. (2022). Measurement of Exhaled Volatile Organic Compounds as a Biomarker for Personalised Medicine: Assessment of Short-Term Repeatability in Severe Asthma. Journal of Personalized Medicine, 12(10), 1635.

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Volatile Compound Analysis by GC-MS

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Samples were collected at the end of fermentation and the volatile substances were analyzed by GC-MS (Agilent 7890B gas chromatography and Agilent 5977A mass spectrometer, Santa Clara, CA, USA), according to the method of Aznar and Arroyo [28 ]. The purge and capture extraction conditions were as follows: sample volume, 5 mL; dilution ratio of water to sample, 1:4 (v/v). The volatile substances were analyzed using the Atomax Teklink software control purging sample concentration system and then passed through N₂ at a rate of 40 mL/min for 11 min at room temperature, with a desorption time of 5 min at 250 °C.
After extraction, the samples were analyzed by GC-MS. The gas chromatographic conditions were as follows: The column was an Agilent J & W DB-624 super-inert capillary column (30 m × 250 μm × 1.4 μm), carrying He gas at a velocity of 1 mL/min. The initial column temperature was kept at 35 °C for 2 min, after which it was raised to 120 °C at a rate of 5 °C/min and then to 220 °C at a rate of 10 °C/min. The column temperature was then kept at 220 °C for 2 min. The MS conditions included an ionization energy of 70 eV and an MS scanning range of 35–550 m/z.

Gao T., Chen J., Xu F., Wang Y., Zhao P., Ding Y., Han Y., Yang J, & Tao Y. (2022). Mixed Mulberry Fruit and Mulberry Leaf Fermented Alcoholic Beverages: Assessment of Chemical Composition, Antioxidant Capacity In Vitro and Sensory Evaluation. Foods, 11(19), 3125.

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GC-MS Analysis of Essential Oil

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Slightly modified from a previous study [16] (link), GC–MS (Agilent 7890B gas chromatography and Agilent 5977 mass spectrometer, CA, USA) was utilized to analyze the chemical component of LCEO. The initial column temperature was set at 50 °C for 2 min, then increased to 120 °C at a rate of 3 °C/min. After 2 min, the temperature was further increased to 250 °C at a rate of 15 °C/min and held for 5 min. The column flow rate was 1 mL/min. The injection, quadrupole, and ionization temperatures were set at 220 °C, 150 °C, and 230 °C, respectively. The photomultiplier voltage was set to 1024 V. The mass scan range was selected from 30 to 550 AMU. Peaks were identified and analyzed using the Nist147 technique library.

Peng Q., Huang Z., Liang G., Bi Y., Kong F., Wang Z., Tan S, & Zhang J. (2024). Preparation of protein-stabilized Litsea cubeba essential oil nano-emulsion by ultrasonication: Bioactivity, stability, in vitro digestion, and safety evaluation. Ultrasonics Sonochemistry, 107, 106892.

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GC-MS and Olfactometry Analysis of Volatile Compounds

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The volatile extract was analysed by a 7890B gas chromatography (Agilent Technologies, USA) equipped with a 5977B electron ionization mass spectrometer (Agilent) and an ODP-3 olfactometer (Gerstel Inc, Germany), according to the method of Yin, Maradza, Xu, Ma, Shi, & Zhao (2022) (link). The volatiles were separated on an HP-5MS capillary column (30 m × 0.25 mm, 0.25 μm) with helium as the carrier gas (purity of 99.999 %). The temperature of the GC column was initially set at 40 °C (held for 5 min), climbed to 230 °C by 3 °C/min (held for 5 min), and increased up to 230 °C by 10 °C/min. The GC effluent was split equally between the ODP and the MSD. The MSD was set as follows: electron bombardment ion source at 230 °C, ionisation energy of 70 eV, full scan mode, the scan range of 33–350 m/z, and the quadrupole at 150 °C. The identifications of odourants were verified by their odour description (O) from three trained panelists (2 females and 1 male) perceived at the ODP, their mass spectra (MS) compared to the NIST17 library, retention indexes (RI), and a comparison to their standard compounds (STD). The relative concentrations of volatile compounds were calculated by the internal standard method (Yin et al., 2022 (link), Yin et al., 2022 (link)).

Zhang F., Wang X.D., Li K., Yin W.T., Liu H.M., Zhu X.L, & Hu P. (2023). Characterisation of flavourous sesame oil obtained from microwaved sesame seed by subcritical propane extraction. Food Chemistry: X, 21, 101087.

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Quantification of Short-Chain Fatty Acids

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The SCFAs quantification method has been described in our previous study (Wang et al., 2020 (link)). Briefly, 10 mg of the powdered intestinal content was accurately weighed and 400 μl of the precooled saturated sodium chloride solution and appropriate internal standard were added to make the concentration of internal standard as 10 μg/ml. 5 μl of 10% H₂SO₄ was added to acidify the system. The sample was homogenized for 10 min and 400 μl of the precooled ethyl acetate was added to it, which was then again homogenized for 10 min and centrifuged at 12,000 rpm and 4°C temperature for 10 min. The supernatant was obtained, and 0.25 g of the anhydrous sodium sulfate was added to the supernatant and centrifuged at 12,000 rpm and 4°C for 10 min. The supernatant was obtained for gas chromatography–mass spectrometry (GC-MS) detection. The determination of SCFAs was performed on 7890B gas chromatography coupled with a 7000D mass spectrometric detector (Agilent Technologies, United States). The SCFAs were separated using a DB-FFAP column (30 m × 0.25 mm, 0.25 μm). The detailed chromatography and mass spectrum conditions are provided in the Supplementary Material. The Agilent MassHunter quantitative analysis software was used to determine the contents of SCFAs in each sample by comparing the peak area of SCFAs and internal standards in each sample.

Shao J., Li Z., Gao Y., Zhao K., Lin M., Li Y., Wang S., Liu Y, & Chen L. (2021). Construction of a “Bacteria-Metabolites” Co-Expression Network to Clarify the Anti–Ulcerative Colitis Effect of Flavonoids of Sophora flavescens Aiton by Regulating the “Host–Microbe” Interaction. Frontiers in Pharmacology, 12, 710052.

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GC/Q-TOF MS Analysis of Essential Oils

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GC/Q-TOF MS analysis was performed on an Agilent quadrupole time of flight mass spectrometer (Agilent Technology Co., LTD, USA) consisting of an Agilent 7890B gas chromatography and an Agilent 7250 time of flight mass spectrometer, using an HP-5MS capillary column (30 m × 250 μm × 0.25 μm film thickness). The operational conditions of GC/Q-TOF MS analysis were preset as follows: helium was used as the carrier gas at 1.0 mL/min flow rate; split ratio 1:20; injection volume 1 μL; injection temperature 230 °C; ion source temperature 230 °C; interface temperature 280 °C; oven temperature progress: 60 °C (holding for 2 min), 60–120 °C (6 °C/min, holding for 2 min), 120–180 °C (5 °C/min), 180–280 °C (30 °C/min); ionization mode used at electronic impact 70 eV; mass range 29–500 m/z; TOF-scan mode at 5 spectra/s; solvent delay 4 min. The mass spectra of essential oil compositions in the NIST14 mass spectral library and the fragmentation patterns of the mass spectra with the data published in the literature were applied for the identification of essential oil compositions.

Liu J.Z., Lyu H.C., Fu Y.J, & Cui Q. (2022). Amomum tsao-ko essential oil, a novel anti-COVID-19 Omicron spike protein natural products: A computational study. Arabian Journal of Chemistry, 15(7), 103916.

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Lipid and Fatty Acid Profiling of Lauraceae

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To analyze the lipid content and FA composition, seed kernels from nine species of Lauraceae family were collected and grinded into fine powders in liquid nitrogen. Lipid extraction was performed as previously described [40 ]. Glyceryl triheptadecanoate (Cat# T2151, Sigma-Aldrich, USA) was added as internal standard (50 μg each sample). The extracted lipids were esterified into FA methyl esters (FAMEs) and analyzed with GC-FID (Agilent 7890B Gas Chromatography equipped with DB23 column, 60m*0.25mm* 0.25 μm, Agilent,USA). The temperature program initiated with 160°C for 1.5 minutes and increased to 240°C at a rate of 20°C/minutes, then kept at 240°C for 10 minutes. The total lipid content and FA compositions were calculated by comparing the peak area of target FAs and the internal standard (methyl heptadecanoate). Data presented are mean ± SD of three biological replicates. The seed oil content and FA composition of U. californica and L. angustifolia were taken from previous published literatures for comparison purposes [37 (link), 41 ].

Gan Y., Song Y., Chen Y., Liu H., Yang D., Xu Q, & Zheng Z. (2018). Transcriptome analysis reveals a composite molecular map linked to unique seed oil profile of Neocinnamomum caudatum (Nees) Merr. BMC Plant Biology, 18, 303.

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