The reperfusion model differs from NMP in that whole autologous blood is used (containing all blood components), and certain protective ingredients are omitted (Table 1). The system has no ability to produce creatinine metabolically; therefore, 1,000 mol creatinine (Sigma-Aldrich, Steinheim, Germany) were added to the circuit to permit creatinine clearance measurement. After 1 h of NMP, the kidneys were removed from the system and flushed with 100 ml of Ringers lactate solution at 4°C to remove blood and cool the organ. Kidneys were then placed on the circuit and reperfused at a near-tonormal (11) RPP of 85 mmHg for 3 h at 38°C. All kidneys were reperfused with a 95% O 2-5% CO2 gas mixture.
Creatinine
Creatinine is a lab equipment product used for the measurement of creatinine levels in biological samples. Creatinine is a waste product formed by the breakdown of creatine, which is found in muscle tissue. The measurement of creatinine levels is commonly used to assess kidney function.
Lab products found in correlation
132 protocols using creatinine
Isolated Organ Perfusion for Renal Assessment
The reperfusion model differs from NMP in that whole autologous blood is used (containing all blood components), and certain protective ingredients are omitted (Table 1). The system has no ability to produce creatinine metabolically; therefore, 1,000 mol creatinine (Sigma-Aldrich, Steinheim, Germany) were added to the circuit to permit creatinine clearance measurement. After 1 h of NMP, the kidneys were removed from the system and flushed with 100 ml of Ringers lactate solution at 4°C to remove blood and cool the organ. Kidneys were then placed on the circuit and reperfused at a near-tonormal (11) RPP of 85 mmHg for 3 h at 38°C. All kidneys were reperfused with a 95% O 2-5% CO2 gas mixture.
Linearity Evaluation of NMR Metabolites
Biochemical Analysis of Animal Samples
Normothermic Machine Perfusion of Donor Kidneys
To provide a direct comparison between CS and NMP in the absence of the ability to transplant these kidneys, ex vivo reperfusion with whole blood was undertaken in paired kidneys to simulate transplantation (MAP 85–95 mm Hg and temperature 37°C).15 (link),23 (link),24 (link) Perfusion parameters, additives, and constituents in both the NMP and ex vivo whole blood reperfusion system are detailed in
Quantitative Analysis of Metabolites
Intestinal Permeability Assay in Mice
Fluorescent Probes for PS Microplastics
Rat Kidney Normothermic Machine Perfusion
Determination of Heterocyclic Amines in TRE
Creatine and Creatinine Riboside Analysis
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