All solvents used in this study were either of HPLC or MS grade, depending on the application. All were purchased from Fisher Scientific. Catharanthine 4 was purchased from Sigma Aldrich, whilst tabersonine 5 was obtained from Ava Chem Scientific. Stemmadenine acetate was synthesized as described before2 . NADPH was from Roche. Carbenicillin was from Formedium. All genes and fragment amplifications were performed using Platinum Superfi polymerase (Thermo Fisher) whilst colony PCRs were performed using Phire II master mix (Thermo Fisher). PCR product purifications were performed using the Macherey-Nagel PCR clean-up kit. Plasmids purifications were performed using Promega Wizard minipreps. Sequencing of the clones was performed by Eurofins Genomics (Germany).
Wizard minipreps
Manufactured by Promega
The Wizard Minipreps is a laboratory equipment product that is designed for the rapid and efficient purification of plasmid DNA from bacterial cultures. It utilizes a silica-based resin to selectively bind and purify plasmid DNA, allowing for quick and reliable DNA isolation.
Automatically generated - may contain errors
2 protocols using wizard minipreps
1
Synthetic Alkaloid Compounds Preparation
2
Synthetic Alkaloid Compounds Preparation
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All solvents used in this study were either of HPLC or MS grade, depending on the application. All were purchased from Fisher Scientific. Catharanthine 4 was purchased from Sigma Aldrich, whilst tabersonine 5 was obtained from Ava Chem Scientific. Stemmadenine acetate was synthesized as described before2 . NADPH was from Roche. Carbenicillin was from Formedium. All genes and fragment amplifications were performed using Platinum Superfi polymerase (Thermo Fisher) whilst colony PCRs were performed using Phire II master mix (Thermo Fisher). PCR product purifications were performed using the Macherey-Nagel PCR clean-up kit. Plasmids purifications were performed using Promega Wizard minipreps. Sequencing of the clones was performed by Eurofins Genomics (Germany).
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