Hy 15763

Manufactured by MedChemExpress

Sourced in United States

HY-15763 is a laboratory equipment product offered by MedChemExpress. It is designed for general laboratory use, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. More information may be available from the manufacturer.

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Lab products found in correlation

5 protocols using hy 15763

Hypoxia-Induced Ferroptosis Regulation

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HTR-8/SVneo cells were obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cells were grown at 37 °C, 5% CO₂.
The si-PPARγ, si-RXRα, si-Nrf2, oe-GPX4, and negative controls (si-NC and oe-NC) were purchased from HonorGene (Changsha, China). The si-PPARγ, si-RXRα, si-Nrf2, oe-GPX4, si-SREBP1, and negative controls were transfected into cells by Lipofectamine 2000. Rosiglitazone (122320-73-4, MedChemExpress, USA) is an agonist of PPARγ. Erastin (HY-15763, MedChemExpress, USA) is an agonist of ferroptosis and ferrostatin-1 (ferr1, HY-100579, MedChemExpress, USA) is an inhibitor. After treatment with 1 μM Rosiglitazone, 1 μM ferr1, and 1 μmol erastin for 24 h, cells were subjected to hypoxia for 24 h. As described earlier^{25 (link)}, the cells were placed under 2% O₂ for 24 h to establish a hypoxic cell model. Cells in the NC group were given normoxic conditions with 20% O₂. Cells were observed by a transmission electron microscope (jem-1400).

Lai W., Yu L, & Deng Y. (2024). PPARγ alleviates preeclampsia development by regulating lipid metabolism and ferroptosis. Communications Biology, 7, 429.

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Erastin, Vitamin C, and Ferroptosis

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Cells (3 × 10³) were seeded into a 96-well culture plate and treated with erastin (HY-15763, MedChemExpress) and/or vitamin C with or without deferoxamine (DFO; D9533, Sigma–Aldrich) for 24 h. To assess the efficiency of cell proliferation, Cell Counting Kit-8 (CCK8, A311-01/02, Vazyme) was used according to the manufacturer's protocol. The absorbance value (OD) was measured with a microplate reader at 450 nm.

Liu Y., Huang P., Li Z., Xu C., Wang H., Jia B., Gong A, & Xu M. (2022). Vitamin C Sensitizes Pancreatic Cancer Cells to Erastin-Induced Ferroptosis by Activating the AMPK/Nrf2/HMOX1 Pathway. Oxidative Medicine and Cellular Longevity, 2022, 5361241.

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Ferroptosis and Alzheimer's Disease

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HT22 cells were seeded in a 96-well plate (5 × 10³ cells per well) and pretreated with FA (40 μM and 80 μM) for 3 h, followed by 24-h co-exposure to 10 μM of erastin (HY-15763, MedChemExpress, Shanghai, China). N2a cells (5 × 10³ cells per well) were seeded in a 96-well plate and pretreated with FA (40 μM and 80 μM) for 3 h, followed by 24-h co-exposure to 10 μM of Aβ oligomers (052487, GL Biochem Ltd., Shanghai, China). Cell viability was measured using 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (S19063, Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China) at 490 nm, as described in our previous study 20 .

Wang C., Chen S., Guo H., Jiang H., Liu H., Fu H, & Wang D. (2022). Forsythoside A Mitigates Alzheimer's-like Pathology by Inhibiting Ferroptosis-mediated Neuroinflammation via Nrf2/GPX4 Axis Activation. International Journal of Biological Sciences, 18(5), 2075-2090.

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Ferroptosis Modulation in HASMCs

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HASMCs were purchased from China Center for Type Culture Collection (CCTCC, GPC0113). Cells were cultured in Dulbecco Modified Eagle medium (DMEM, Hyclone) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Hyclone) in an incubator with 5% CO₂ at 37°C. After reaching a density of 70–80%, cells were treated with erastin (HY-15763, MedChemExpress), zVAD-fmk (HY-16658B, MedChemExpress), necrosulfonamide (HY-100573, MedChemExpress), 3-Methyladenine (3MA, HY-19312, MedChemExpress), Zinc Protoporphyrin (ZnPP, HY-101193, MedChemExpress), hemin (HY-19424, MedChemExpress) and Deferoxamine (DFO, Ba33112, MedChemExpress) for 24 h, respectively. Human monocytic cell line THP-1 was acquired from CCTCC (GDC0100) and cultured in Roswell Park Memorial Institute 1640 medium (RPMI 1640; Hyclone) supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Hyclone) in an incubator with 5% CO₂ at 37°C.

Wu D., Hu Q., Wang Y., Jin M., Tao Z, & Wan J. (2022). Identification of HMOX1 as a Critical Ferroptosis-Related Gene in Atherosclerosis. Frontiers in Cardiovascular Medicine, 9, 833642.

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Knockdown of TFRC Induces Ferroptosis in HUVECs

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HUVECs were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences and cultured in Dulbecco's modified Eagle's medium supplemented with 10 % fetal bovine serum and 1 % (v/v) penicillin/streptomycin in a 37 °C incubator with a humidified atmosphere of 5 % CO₂. The passage number of the cell lines was approximately F3∼F5.
A shRNA targeting TFRC was synthesized by Tsingke Biotechnology Co., Ltd. (Beijing, China), and the sequence that was used for interference was as follows: GCTGGTCAGTTCGTGATTAAA. The vector structure is shown in Fig. S4C. HUVECs were transfected with TFRC shRNA or negative control shRNA using polybrene reagent. The cells were transduced with lentivirus (multiplicity of infection = 100). After three days, stable clones expressing the shRNA were selected via puromycin dihydrochloride (MedChemExpress, China). The medium was replaced with new puromycin-supplemented medium every 2–3 days until resistant colonies were selected. The resistant colonies were expanded, and GFP fluorescence and Western blotting analyses were performed to evaluate stable shRNA transduction and knockdown efficiency. Normal cells and transinfected cells were treated with DMSO (vehicle reagent), erastin (ferroptosis agonist, HY-15763; MedChemExpress), or deferoxamine mesylate (iron chelator, DFOM; HY-B0988; MedChemExpress) for 24 h.

Ma H., Huang Y., Tian W., Liu J., Yan X., Ma L, & Lai J. (2024). Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis. Redox Biology, 70, 103041.

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