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Xm10 fluorescent camera

Manufactured by Olympus
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The XM10 Olympus fluorescent camera is a high-quality imaging device designed for use in scientific and research applications. It features a sensitive CMOS sensor that captures fluorescent signals with high resolution and low noise. The camera is capable of capturing live, real-time images and videos, making it a valuable tool for a wide range of laboratory studies and experiments.

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Cellsens software, by Olympus (1 mentions)

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XM10 camera (61 citations)

2 protocols using xm10 fluorescent camera

1

Epididymal Adipocyte Morphometry Analysis

Cited 1 time
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Epididymal (eWAT) depot was fixed in 4% paraformaldehyde for 48 h, embedded in paraffin, cut into 5-μm sections, and stained with Hematoxylin and Eosin (H&E). The adipocyte diameter was determined as previously described (Wheatcroft et al., 2007 (link)). The total number of adipocytes was measured using the CellSens Software. The images were acquired with an XM10 Olympus fluorescent camera.
Buffolo M., Pires K.M., Ferhat M., Ilkun O., Makaju A., Achenbach A., Bowman F., Atkinson D.L., Holland W.L., Amri E.Z., Chaurasia B., Franklin S, & Boudina S. (2019). Identification of a Paracrine Signaling Mechanism Linking CD34high Progenitors to the Regulation of Visceral Fat Expansion and Remodeling. Cell reports, 29(2), 270-282.e5.
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2

Quantitative Histological Analysis of Adipose Tissue

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Epididymal (eWAT) depot and interscapular brown adipose tissue (BAT) were fixed in 4% paraformaldehyde for 48 hr, embedded in paraffin, cut into 5-mm sections, and stained with Hematoxylin and Eosin (H&E). The total number of adipocytes was measured using the CellSens Software (Olympus). Adipocyte number per area was averaged from at least 500 adipocytes counted from 12–15 images per animal. Crown-like structures were counted on paraffin-embedded and H&E stained eWAT sections. Paraffin-embedded eWAT sections were stained with an anti-F4/80 antibody to detect macrophages in the adipose tissue. The images were acquired with an XM10 Olympus fluorescent camera (Olympus). F4/80 immunofluorescence staining was quantified based on color intensity using the CellSens Software (Olympus) in representative nonconsecutive fields per slide from three slides per animal in a blinded fashion.
Cai J., Pires K.M., Ferhat M., Chaurasia B., Buffolo M.A., Smalling R., Sargsyan A., Atkinson D.L., Summers S.A., Graham T.E, & Boudina S. (2018). Autophagy Ablation in Adipocytes Induces Insulin Resistance and Reveals Roles for Lipid Peroxide and Nrf2 Signaling in Adipose-Liver Crosstalk. Cell reports, 25(7), 1708-1717.e5.
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