Glutamine

Manufactured by Sartorius

Sourced in Israel, United States

Glutamine is a laboratory reagent used in cell culture media. It serves as a primary source of nitrogen and energy for cells, supporting their growth and proliferation.

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Lab products found in correlation

Streptomycin, by Sartorius (32 mentions) Penicillin, by Sartorius (31 mentions) Fetal bovine serum (fbs), by Sartorius (26 mentions) Penicillin streptomycin, by Sartorius (19 mentions) Sodium pyruvate, by Sartorius (12 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (11 mentions) Hepes, by Sartorius (9 mentions) Dulbecco modified eagle medium (dmem), by Sartorius (8 mentions) Rpmi 1640 medium, by Sartorius (8 mentions) Rpmi 1640, by Sartorius (6 mentions) Non essential amino acids (neaa), by Sartorius (6 mentions) Fetal calf serum (fcs), by Sartorius (6 mentions) 2 deoxy d glucose, by Merck Group (5 mentions) Penicillin, by Merck Group (4 mentions) Streptomycin, by Merck Group (4 mentions) Rotenone, by Merck Group (4 mentions) Dulbecco s modified eagle medium, by Sartorius (4 mentions) Penicillin streptomycin solution, by Sartorius (3 mentions) Anti human cd3, by Thermo Fisher Scientific (3 mentions) Roswell park memorial institute (rpmi), by Sartorius (3 mentions)

Close spelling variants of this product

Glucose and glutamine L‐alanine‐l‐glutamine Penicillin/streptomycin/L-glutamine Penicillin-streptomycin-glutamine Stable glutamine L-alanyl-l-glutamine L-glutamine RPMI-1640 without phenol red and L-glutamine L-glutamine solution

79 protocols using glutamine

Cell Culture Maintenance Protocols

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K562, RAJI, 8866, C1R and 721.221 cells were maintained in RPMI (Sigma-Aldrich) supplemented with 10% fetal calf serum (Sigma-Aldrich), 2 mM glutamine (Biological Industries (BI)), 1 mM sodium pyruvate (BI), 1× nonessential amino acids (BI), 100 U/ml penicillin (BI), 0.1 mg/ml streptomycin (BI).
RKO, T-47D, and A549 cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10% fetal calf serum (Sigma-Aldrich), 2 mM glutamine (Biological Industries (BI)), 1 mM sodium pyruvate (BI), 1× nonessential amino acids (BI), 100 U/ml penicillin (BI), 0.1 mg/ml streptomycin (BI).
NK-92 cells were maintained in two different media: RPMI (Sigma-Aldrich) supplemented with 10% fetal calf serum (Sigma-Aldrich), 2 mM glutamine (Biological Industries (BI)), 1 mM sodium pyruvate (BI), 1× nonessential amino acids (BI), 100 U/ml penicillin (BI), 0.1 mg/ml streptomycin (BI), and 200U/ml IL-2 (PeproTech); or MEM-alpha (BI) supplemented with 12.5% fetal calf serum (Sigma-Aldrich), 12.5% Horse serum (BI), 2 mM glutamine (BI), 1 mM sodium pyruvate (BI), 1× nonessential amino acids (BI), 100 U/ml penicillin (BI), 0.1 mg/ml streptomycin (BI), 200 U/ml IL-2 (PeproTech), 0.2 mM myoinositol, 0.02 mM folic acid, 0.1mM beta-mercaptoethanol, 0.2% Ribonucleosides and Deoxyribonucleosides for MEM-Alpha.

Kotzur R., Duev-Cohen A., Kol I., Reches A., Mandelboim O, & Stein N. (2022). NK-92 cells retain vitality and functionality when grown in standard cell culture conditions. PLoS ONE, 17(3), e0264897.

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Culturing Human Osteosarcoma Cell Lines

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Human OS cell lines U2-OS and 143B (ATCC, Gaithersburg, MD, USA) were cultured according to the manufacturer’s instructions. U2-OS cells were cultured in low glucose Dulbecco’s Modified Eagle Medium (Low DMEM) supplemented with 10% FBS, 1% penicillin–streptomycin–amphotericin B, and 1% glutamine (Biological Industries Ltd.). 143B cells were cultured in Minimum Essential Medium Eagle (MEM-Eagle) with 0.015 mg/mL 5-bromo-2′deoxyuridine (SIGMA-ALDRICH, Burlington, MA, USA) supplemented with 10% FBS, 1% penicillin–streptomycin–amphotericin B, and 1% glutamine (Biological Industries Ltd.). Cells were cultured at 37 °C in a humidified atmosphere of 95% air/5% CO₂. Cells were fed twice a week, and when reachinhg 80–90% confluence, they were split by trypsinization, using a solution containing 0.5% trypsin in 0.25% ethylenediaminetetraacetic acid (EDTA) (Biological Industries Ltd.).

Doppelt-Flikshtain O., Younis A., Tamari T., Ginesin O., Shentzer-Kutiel T., Nikomarov D., Bar-Sela G., Coyac B.R., Assaraf Y.G, & Zigdon-Giladi H. (2023). Endothelial Progenitor Cells Promote Osteosarcoma Progression and Invasiveness via AKT/PI3K Signaling. Cancers, 15(6), 1818.

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Culturing UM cell lines for research

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92-1, OMM2.5, and Mel270 UM cell lines [66 (link)–68 (link)] (kind gift of Prof. Martine Jager, MD, PhD, Leiden University) were grown in RPMI-1640 Dutch Modified medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Biological Industries, Kibbutz Beit-Haemek, Israel), 2 mml-glutamine (Biological Industries, Kibbutz Beit-Haemek, Israel), and 2% penicillin/streptomycin (Biological Industries, Kibbutz Beit-Haemek, Israel). MP46 UM cell line [69 (link)] (also a kind gift of Prof. Martine Jager, MD, PhD, Leiden University) was grown in IMDM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) medium supplemented with 20% FBS (Biological Industries, Kibbutz Beit-Haemek, Israel), 3 mml-glutamine (Biological Industries, Kibbutz Beit-Haemek, Israel), and 2% penicillin/streptomycin (Biological Industries, Kibbutz Beit-Haemek, Israel). The cells were incubated in a cell culture incubator at 37°C, 5% CO₂, and 60% humidity. The cell cultures were maintained by the replacement of media 2–3 times per week. Cells growing as a monolayer were subcultured by trypsin-EDTA once a week.

Deliktas O., Gedik M.E., Koc I., Gunaydin G, & Kiratli H. (2023). Modulation of AMPK Significantly Alters Uveal Melanoma Tumor Cell Viability. Ophthalmic Research, 66(1), 1230-1244.

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Cell Culture Protocol for GM12878, A549, and 293T

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GM12878 lymphoblast cell line (Coriell Institute) was grown in RPMI 1640 Medium without phenol red (01-103-1A; Biological Industries) supplemented with 15% FBS (10270106; Gibco, Rhenium Research Laboratory Equipment Ltd.), 4 mM glutamine (03-020-1B; Biological Industries), and 100 U/ml penicillin, 0.25 mg/ml streptomycin (03-031-1B; Biological Industries). A549 (ATCC CCL-185) and 293T cells were grown in DMEM medium (01-055-1A; Biological Industries) supplemented with 10% FBS, 2 mM glutamine, 1 mM sodium pyruvate (03-042-1B; Biological Industries), 100 U/ml penicillin, and 0.25 mg/ml streptomycin. Mycoplasma was monitored every 3–4 mo.

Merav M., Bitensky E.M., Heilbrun E.E., Hacohen T., Kirshenbaum A., Golan-Berman H., Cohen Y, & Adar S. (2024). Gene architecture is a determinant of the transcriptional response to bulky DNA damages. Life Science Alliance, 7(3), e202302328.

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Antibody Preparation for GFP Detection

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Rabbit antibodies to GFP (FL) (sc-8334) were from Santa Cruz Biotechnology (Santa Cruz, CA). The anti-GFP (FL) antibody served for blotting, while for immunoprecipitation a sheep anti-GFP antibody was used; this antibody, described earlier (Laude and Prior, 2008 (link)), was a generous gift from I. A. Prior, University of Liverpool, UK. Mouse anti-β-actin (antibody 4) was from MP Biomedicals (Santa Ana, CA), and peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG; catalogue number 111-035-003) from Jackson ImmunoResearch Laboratories (West Grove, PA). Immobilized Protein A and GammaBind-Sepharose were from GE Healthcare (Little Chalfont, UK). [9,10–³H]Palmitic acid and [9,10–³H]myristic acid, 30–60 Ci (1.11–2.22 TBq)/mmol each, were from Hartmann Analytic (Braunschweig, Germany). Protease inhibitor cocktail (P8340), Na₃VO₄, and the palmitoylation inhibitor 2-bromopalmitate (2BP) were from Sigma-Aldrich (St. Louis, MO). Restriction enzymes and T4 DNA ligase were from New England Biolabs (Ipswich, MA). Hank’s balanced salts solution (HBSS), DMEM, fetal calf serum (FCS), penicillin-streptomycin solution and glutamine were purchased from Biological Industries (Beit Haemek, Israel). All other reagents were from Sigma-Aldrich.

Gottlieb-Abraham E., Gutman O., Pai G.M., Rubio I, & Henis Y.I. (2016). The residue at position 5 of the N-terminal region of Src and Fyn modulates their myristoylation, palmitoylation, and membrane interactions. Molecular Biology of the Cell, 27(24), 3926-3936.

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Cultivation and Transfection of Ovarian Cancer Cell Lines

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ES-2 human ovarian cancer cells (CRL1978; American Type Culture Collection) were grown in DMEM supplemented with 10% fetal calf serum (FCS), penicillin (25 μg/ml), streptomycin (40 μg/ml), and glutamine (5 mM), all from Biological Industries. Caov3 cells (HTB75; American Type Culture Collection) were grown in DMEM supplemented with 20% FCS, 1 mM sodium pyruvate. and the medium supplements mentioned earlier. ES-2-Dab2 cells were generated from ES-2 cells by stable expression of a myc-tagged rat Dab2 (p82) construct mutated at Asp 227 to Ser to better resemble the human protein. They were maintained in the same growth medium as ES-2 supplemented with puromycin (2 ng/ml) and neomycin (1.5 mg/ml).
Transient transfections were carried out using jetPRIME (Polyplus, Illkirch, France).

Shapira K.E., Hirschhorn T., Barzilay L., Smorodinsky N.I., Henis Y.I, & Ehrlich M. (2014). Dab2 inhibits the cholesterol-dependent activation of JNK by TGF-β. Molecular Biology of the Cell, 25(10), 1620-1628.

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Cell Culture Media and Reagents

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Complete Medium (medium): RPMI-1640, DMEM medium, heat-inactivated fetal calf serum (FCS), penicillin, streptomycin, glutamine, sodium pyruvate and HEPES obtained from Biological Industries (Kibbutz Beit Haemek, Israel). Rotenone and 2-deoxy-d-glucose from Sigma-Aldrich (St. Louis, MO, USA).

Wohl I, & Sherman E. (2019). ATP-Dependent Diffusion Entropy and Homogeneity in Living Cells. Entropy, 21(10), 962.

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Cell Culture and Protein Reagents

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Complete Medium (medium): RPMI-1640, DMEM medium, heat-inactivated fetal calf serum (FCS), penicillin, streptomycin, glutamine, sodium pyruvate, and HEPES obtained from Biological Industries (Kibbutz Beit Haemek, Israel). Mibefradil from Sigma-Aldrich (St. Louis, MO, USA). CD45 proteins were purchased from BioLegend (San Diego, CA, USA). Anti-human CD3 from eBioscience Inc. (ThermoFisher Scientific, Waltham, MA, USA).

Wohl I., Sajman J, & Sherman E. (2023). Cell Surface Vibrations Distinguish Malignant from Benign Cells. Cells, 12(14), 1901.

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Cell Culture Maintenance Protocol

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K562, BCBL1, YTS, P815, Jurkat, BJAB, RAJI, BW, 8866, EL-4 and 721.221 cells were maintained in RPMI (Sigma-Aldrich) supplemented with 10% fetal calf serum (Sigma-Aldrich), 2 mM glutamine (Biological Industries (BI)), 1 mM sodium pyruvate (BI), 1× nonessential amino acids (BI), 100 U/ml penicillin (BI), 0.1 mg/ml streptomycin (BI).
For NK-92 cells the same medium and conditions were used, but 200U/ml IL-2 (PeproTech) were added to the medium.

Kotzur R., Stein N., Kahlon S., Berhani O., Isaacson B, & Mandelboim O. (2023). Eradication of CD48-positive tumors by selectively enhanced YTS cells harnessing the lncRNA NeST. iScience, 26(8), 107284.

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Isolation of Primary Osteoblasts from Mouse Calvaria

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Primary osteoblasts were isolated from mice calvariae as previously described [36 (link)]. All procedures were approved by the Ethics Committee for Animal Experimentation of the Generalitat de Catalunya. Briefly, the calvariae were dissected from P1–P4 pups, and soft tissue was discarded. A total of five to eight calvariae were pooled and digested in α-Minimum Essential Medium Eagle Alpha Modification (α-MEM; SIGMA, St. Louis, MO, USA, M8042) containing trypsin (0.025%)/collagenase II (1 mg/mL; Thermo Fisher Scientific, Waltham, MA, USA, 17101015). The product of the first 5 minutes of digestion was discarded, while the product of a double 20-minute digestion was centrifuged and seeded on 60 mm culture plates. Cells were used between passages 1 to 4. Osteoblasts were cultured at 37 ºC in osteogenic media ((α-MEM with 10% FBS (Biological Industries, 04-007-1A), 2 mM glutamine (Biological Industries, 03-020-1B), 1 mM pyruvate (Biological Industries, 03-042-1B), 100 U/mL penicillin and 0.1 mg/mL streptomycin (Biological Industries, 03-031-1B), 50 µg/mL ascorbic acid and 4 mM β-glycerophosphate).

Simon-Molas H., Sánchez-de-Diego C., Navarro-Sabaté À., Castaño E., Ventura F., Bartrons R, & Manzano A. (2022). The Expression of TP53-Induced Glycolysis and Apoptosis Regulator (TIGAR) Can Be Controlled by the Antioxidant Orchestrator NRF2 in Human Carcinoma Cells. International Journal of Molecular Sciences, 23(3), 1905.

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