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Ab86863

Manufactured by Abcam
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Sourced in Japan

Ab86863 is a lab equipment product from Abcam. It is a tool used for scientific research purposes. The core function of this product is to facilitate specific laboratory procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ab63766, by Abcam (1 mentions) Ab130275, by Abcam (1 mentions) Ab169538, by Abcam (1 mentions) Ab89016, by Abcam (1 mentions) Ab20666, by Abcam (1 mentions) Ab21686, by Abcam (1 mentions) Ab42080, by Abcam (1 mentions) Ab6046, by Abcam (1 mentions) Anti rb, by Cell Signaling Technology (1 mentions) Anti mdm2, by Abcam (1 mentions) Anti myc tag, by Cell Signaling Technology (1 mentions) Anti actin, by Merck Group (1 mentions) Anti phospho rb, by Cell Signaling Technology (1 mentions) Anti flag m2, by Merck Group (1 mentions)

2 protocols using ab86863

1

Antibodies for Immunoblotting, IP, and IF

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Antibodies used for immunoblotting, IP, or immunofluorescence were as follows: anti-TAF7 (TAF7 monoclonal antibody M01, clone 2C5, Abnova); anti-TBP (ab63766, Abcam); anti–β-tubulin (ab6046, Abcam); anti-HA (16B12, ab130275, Abcam); anti-FLAG (clone M2, F3165, MilliporeSigma); anti-puromycin (clone 12D10, MABE343, MilliporeSigma); anti-RPL5 (ab86863, Abcam); anti-RPL8 (ab169538, Abcam); anti-XPO1 (M01180, Boster); anti-eIF5B (ab89016, Abcam); anti-hnRNP U (ab20666, Abcam); anti-Haspin (ab21686, Abcam); anti-SMARCC2 (A301-039A, Bethyl); and anti-DPP9 (ab42080, Abcam). The specificity of the anti-TAF7 antibody for TAF7 has been shown previously using MEFs deleted of TAF7 (13 (link)).
Cheng D., Semmens K., McManus E., Chen Q., Meerzaman D., Wang X., Hafner M., Lewis B.A., Takahashi H., Devaiah B.N., Gegonne A, & Singer D.S. (2021). The nuclear transcription factor, TAF7, is a cytoplasmic regulator of protein synthesis. Science Advances, 7(50), eabi5751.
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2

Antibody Validation for Protein Analyses

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The following antibodies were used in this study: anti-DDX41 (ab182007; Abcam, Cambridge, UK), anti-Actin (MAB1501; Merck Millipore, Darmstadt, Germany), anti-FLAG M2 (F3165; Sigma-Aldrich, St. Louis, MO), anti-Myc-tag (2272; Cell Signaling Technology, Danvers, MA), anti-Nucleolin (M019-3S; MBL, Nagoya, Japan), anti-RPL5 (ab86863; Abcam), anti-RPL11 (ab79352; Abcam), anti-RB (9313; Cell Signaling Technology), anti-phospho-RB (9301; Cell Signaling Technology), anti-MDM2 (ab3110; Abcam), and anti-multi-ubiquitin (D058-3; MBL).
Kadono M., Kanai A., Nagamachi A., Shinriki S., Kawata J., Iwato K., Kyo T., Oshima K., Yokoyama A., Kawamura T., Nagase R., Inoue D., Kitamura T., Inaba T., Ichinohe T, & Matsui H. (2016). Biological implications of somatic DDX41 p.R525H mutation in acute myeloid leukemia. Experimental hematology, 44(8).
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