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Cd11a m17 4

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CD11a (M17/4) is a monoclonal antibody that recognizes the alpha subunit of the CD11a/CD18 integrin complex, also known as Lymphocyte Function-associated Antigen 1 (LFA-1). This antibody can be used for the identification and analysis of cells expressing the CD11a antigen.

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Lab products found in correlation

Tlr4 sa15 21, by BioLegend (2 mentions) Cd3ε 145 2c11, by BioLegend (2 mentions) Nk1.1 pk136, by Thermo Fisher Scientific (2 mentions) Nkp46 29a1.4, by BioLegend (2 mentions) F4 80 bm8, by BioLegend (2 mentions) Cd19 6d5, by BioLegend (2 mentions) Ly6g 1a8, by BioLegend (2 mentions) Cd127 a7r34, by BioLegend (2 mentions) Ly6c hk1, by BioLegend (2 mentions) Cd8α 53 6.7, by BioLegend (2 mentions) Cd4 gk1, by BioLegend (2 mentions) Cytofix solution, by BD (2 mentions) Cd11c hl3, by BD (2 mentions) Tlr2 cb225, by BD (2 mentions) Aqua live dead dye, by Thermo Fisher Scientific (1 mentions) Lsr 2, by BD (1 mentions)

Spelling variants of this product

CD11a (7 citations) CD11a (clone M17/4, (4 citations) Anti- mouse CD11a (clone M17/4) (2 citations) Anti-CD11a (M17/4), (2 citations)

3 protocols using cd11a m17 4

1

Multiparametric Immune Cell Profiling

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Peripheral blood was collected retro-orbitally from isoflurane-anesthetized mice. The blood was treated with ACK lysis buffer for 5 min to remove RBCs, leaving the peripheral blood leukocytes (PBLs). To determine expression of cell surface molecules, we incubated PBLs with mAb at 4°C for 20–30 min, and cells were subsequently fixed for 10 min using Cytofix Solution (BD Biosciences). The following mAb clones were used to stain processed samples: CD11a (M17/4; eBioscience), TLR4 (SA15-21; BioLegend), CD3ε (145-2C11; BioLegend), NK1.1 (PK136; eBioscience), NKp46 (29A1.4; BioLegend), F4/80 (BM8; BioLegend), TLR2 (CB225; BD Bioscience), CD19 (6D5; BioLegend), CD11c (HL3; BD Biosciences), CD4 (GK1.5; BioLegend), Ly6G (1A8; BioLegend), CD127 (A7R34; BioLegend), Ly6C (HK1.4; BioLegend), and CD8α (53-6.7; BioLegend). Flow cytometry data were acquired on a Cytek Aurora (Cytek, Bethesda, MD) and analyzed with FlowJo software (Tree Star, Ashland, OR).
Berton R.R., Jensen I.J., Harty J.T., Griffith T.S, & Badovinac V.P. (2022). Inflammation Controls Susceptibility of Immune-Experienced Mice to Sepsis. ImmunoHorizons, 6(7), 528-542.
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2

Single cell analysis of lymph nodes

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Single cell suspensions were prepared from mesenteric lymph nodes of Ncr1GFP/+ mice 5 days after oral infection with tdTomato-expressing parasites. Cells were stained with a fixable Aqua Live/Dead dye (Molecular Probes), then with antibodies to mouse CD3ε (145-2C11, Ebioscience) and CD11a (M17/4, Ebioscience). Data was acquired using a BD LSR II and analyzed with FlowJo software.
Ueno N., Lodoen M.B., Hickey G.L., Robey E.A, & Coombes J.L. (2014). Toxoplasma gondii-infected natural killer cells display a hypermotility phenotype in vivo. Immunology and Cell Biology, 93(5), 508-513.
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3

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood was collected retro-orbitally from isoflurane-anesthetized mice. The blood was treated with ACK lysis buffer for 5 min to remove RBCs, leaving the peripheral blood leukocytes (PBLs). To determine expression of cell surface molecules, we incubated PBLs with mAb at 4°C for 20–30 min, and cells were subsequently fixed for 10 min using Cytofix Solution (BD Biosciences). The following mAb clones were used to stain processed samples: CD11a (M17/4; eBioscience), TLR4 (SA15-21; BioLegend), CD3ε (145-2C11; BioLegend), NK1.1 (PK136; eBioscience), NKp46 (29A1.4; BioLegend), F4/80 (BM8; BioLegend), TLR2 (CB225; BD Bioscience), CD19 (6D5; BioLegend), CD11c (HL3; BD Biosciences), CD4 (GK1.5; BioLegend), Ly6G (1A8; BioLegend), CD127 (A7R34; BioLegend), Ly6C (HK1.4; BioLegend), and CD8α (53-6.7; BioLegend). Flow cytometry data were acquired on a Cytek Aurora (Cytek, Bethesda, MD) and analyzed with FlowJo software (Tree Star, Ashland, OR).
Berton R.R., Jensen I.J., Harty J.T., Griffith T.S, & Badovinac V.P. (2022). Inflammation Controls Susceptibility of Immune-Experienced Mice to Sepsis. ImmunoHorizons, 6(7), 528-542.
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