For GNPs isolation, we followed the previously described protocol (Li et al., 2013 (link)), cerebella were harvested from C57BL/6J mice at P4-P7 then digested in a solution containing 10 units/ml papain (Worthington), 25 U/ml DNase I (Solarbio) and 2 mg L-cysteine (Solarbio) at 37°C for 30 min to obtain a single-cell suspension. After filtered with 70 μm strainer, cells were centrifuged through a 35%–65% Percoll gradient (GE Healthcare). Cells from the 35–65% interface were suspended in Neurobasal Medium with B27 supplement. Then GNPs were suspended in NB-B27 and plated on Poly-L-omithine hydrobromide (PLO) and Laminin (all from Sigma)-coated coverslips. GNPs was treated with 5 μM PTL, 50% sonic hedgehog conditional medium (Shh-CM) and Shh-CM adding 5 μM PTL for 24 h. EDU (Proteintech) was added 20 μM and incubated GNPs for 1 h. Then stained according to the instructions.
L cysteine
Manufactured by Solarbio
Sourced in China
L-cysteine is an amino acid that serves as a building block for proteins. It is a white crystalline powder with a characteristic odor.
Automatically generated - may contain errors
Lab products found in correlation
Test kit, by Highfine Biotech (3 mentions)
Laminin, by Merck Group (1 mentions)
Dnase 1, by Solarbio (1 mentions)
Percoll gradient, by GE Healthcare (1 mentions)
Papain, by Worthington (1 mentions)
Sodium borohydride nabh4, by Merck Group (1 mentions)
L glutathione reduced gsh, by Merck Group (1 mentions)
Agno3, by Merck Group (1 mentions)
Cystine, by Solarbio (1 mentions)
Isoleucine, by Solarbio (1 mentions)
Methionine, by Solarbio (1 mentions)
Glutamine, by Solarbio (1 mentions)
Proline, by Solarbio (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
2 chloro l phenylalanine, by Adamas (1 mentions)
2 propanol, by Merck Group (1 mentions)
Glycine, by Solarbio (1 mentions)
Lysine, by Solarbio (1 mentions)
Tyrosine, by Solarbio (1 mentions)
8 protocols using l cysteine
1
Isolation and Culture of Cerebellar GNPs
2
Synthesis of Silver Nanoparticles
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Milli-Q water (18.2 MΩ.cm/25 °C) was used throughout this study. All glassware were washed with 0.05 M nitric acid, rinsed with Milli-Q water, and dried in an oven overnight. AgNO3, (99.9999%), L-glutathione reduced (GSH) and sodium borohydride (NaBH4) were purchased from Sigma-Aldrich. L-cysteine (≥ 99%) was purchased from Beijing Solarbio Science & Technology Co., Ltd. The other reagents (analytical reagent) were purchased from China National Pharmaceutical Group Corporation.
3
Ziziphus jujuba Leaf Metabolite Profiling
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Leaves from the Ziziphus jujuba Mill. cv. Dongzao were used, and the picking standard is two leaves and one bud on the fruit-bearing shoot (Figure 1C
4
Profiling Fungal Bioactive Compounds
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Fresh Yu Muer fruiting bodies were collected from the Fungus and Vegetable Base, Jilin Agricultural University (Changchun City, Jilin Province, China). Test kits for determining PPO, amino acid (AA) content, plant total phenolics, protein content (BCA assay), and total sugar content were purchased from Suzhou Keming Biotechnology Co., Ltd. Sodium erythorbate and L‐cysteine were purchased from Beijing Solarbio Science and Technology Co., Ltd. Ascorbic acid was purchased from Shanghai Yuanye Biotechnology Co., Ltd. Citric acid was purchased from Tianjin Fuchen Chemical Reagent Factory.
5
Antimicrobial Susceptibility of M. hyopneumoniae
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A standard strain of M. hyopneumoniae (ATCC 25934) was obtained from the Chinese Veterinary Microorganism Culture Collection Center (Beijing, China) and stored at -80 ℃. Tilmicosin (75.8%), tylosin (82.6%), erythromycin (85.0%), tiamulin (99.0%), doxycycline (85.8%), and enrofloxacin (99.0%) were kindly supplied by Guangdong Dahuanong Animal Health Products (Xincheng, China). Amikacin (99.0%) and lincomycin (84.6%) were purchased from Guangdong Puboxing Animal Health Products (Guangzhou, China) and stored at -80 C before use.
A fresh stock solution (1280 mg/L) of each antibacterial agent was prepared for each experiment. Broth medium base was purchased from Qingdao Hope Biological Technology (Qingdao, China). The reduced form of nicotinamide adenine dinucleotide was obtained from Beijing Newprobe Biotechnology (Beijing, China). L-Cysteine was purchased from Beijing Solarbio Science and Technology (Beijing, China).
A fresh stock solution (1280 mg/L) of each antibacterial agent was prepared for each experiment. Broth medium base was purchased from Qingdao Hope Biological Technology (Qingdao, China). The reduced form of nicotinamide adenine dinucleotide was obtained from Beijing Newprobe Biotechnology (Beijing, China). L-Cysteine was purchased from Beijing Solarbio Science and Technology (Beijing, China).
6
Microbial Sensitivity Testing of M. hyopneumoniae
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A standard strain of M. hyopneumoniae (ATCC 25934) was obtained from the Chinese Veterinary Microorganism Culture Collection Center (Beijing, China) and stored at −80 °C. Tilmicosin (75.8%), tylosin (82.6%), erythromycin (85.0%), tiamulin (99.0%), doxycycline (85.8%) and enrofloxacin (99.0%) were kindly supplied by Guangdong Dahuanong Animal Health Products (Xincheng, China). Amikacin (99.0%) and lincomycin (84.6%) were purchased from Guangdong Puboxing Animal Health Products (Guangzhou, China) and stored at −80 °C before use.
A fresh stock solution (1280 mg/L) of each antibacterial agent was prepared for each experiment. Broth medium base was purchased from Qingdao Hope Biological Technology (Qingdao, China). The reduced form of nicotinamide adenine dinucleotide was obtained from Beijing Newprobe Biotechnology (Beijing, China). L-Cysteine was purchased from Beijing Solarbio Science and Technology (Beijing, China).
A fresh stock solution (1280 mg/L) of each antibacterial agent was prepared for each experiment. Broth medium base was purchased from Qingdao Hope Biological Technology (Qingdao, China). The reduced form of nicotinamide adenine dinucleotide was obtained from Beijing Newprobe Biotechnology (Beijing, China). L-Cysteine was purchased from Beijing Solarbio Science and Technology (Beijing, China).
7
Extraction and Analysis of Dry Tubers
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Dry B. striata tubers were bought from Hubei Zexi Traditional Chinese Medicine Technology Co., Ltd. (Qichun, Hubei, China). The authenticity of medicinal materials was identified by Dr. Xiongjie Sun of Hubei University of Chinese Medicine. Vitamins, yeast extract, peptone, bacterial DNA extraction kit, bile salts, and L-cysteine were obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Pepsin, α-amylase, pancreatin, trypsin, and SCFAs standards (Acetic acid, propionic acid, butyric acid, pentanoic acid, and indole) were bought from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). All materials were of standard analytical grades.
8
Cultivation and Preparation of Bacterial Strains
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Bifidobacterium bifidum WBBI03 was provided by the Jiangxi-Oai Joint Research Institute, Nanchang University, Nanchang, P. R. China. Listeria monocytogenes CMCC 54001 was provided by the National Center for Medical Culture Collection (CMCC) of China. A single colony of L. monocytogenes was subcultured aerobically in brain heart infusion (BHI) broth (Beijing Solarbio Science and Technology Co. Ltd., Beijing, China) under shaking (180 rpm) at 37°C for 18 h. Bifidobacterium bifidum was cultured under anaerobic conditions in de Man, Rogosa, and Sharpe (MRS) broth (Difco Laboratories, Detroit, MI) containing 0.05% l-cysteine (Beijing Solarbio Science and Technology Co. Ltd.) at 37°C for 24 h. The bacterial suspension was centrifuged at 8,000 × g for 10 min at 4°C. After that, the bacteria were washed 3 times with PBS and resuspended in PBS. The cell concentration was adjusted to approximately 10 9 cfu/mL according to the corresponding curve of absorbance and the viable count.
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