Male lewis rats

Manufactured by Charles River Laboratories

Sourced in Germany, United States, China, Japan, Canada

Male Lewis rats are a strain of laboratory rats commonly used in biomedical research. They are characterized by their albino appearance and are known for their docile temperament and reliable breeding performance.

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Lab products found in correlation

C57bl 6j mice, by Charles River Laboratories (1 mentions) Male wistar rats, by Charles River Laboratories (1 mentions) Complete freund s adjuvant, by BD (1 mentions) Crf 1, by Oriental Yeast (1 mentions) Buprenorphine hydrochloride, by Reckitt Benckiser (1 mentions) Lab diet 5001, by Purina (1 mentions) Rotenone, by Merck Group (1 mentions) Standard chow, by Altromin (1 mentions) Lv bmp 2, by Merck Group (1 mentions) Polybrene, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Omega Scientific (1 mentions) Porcine cardiac myosin, by Merck Group (1 mentions) Medisafe automatic analyzer, by Terumo (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) Sprague dawley rat, by Charles River Laboratories (1 mentions) Pico rodent diet 20 ext irr, by LabDiet (1 mentions)

Spelling variants of this product

Lewis rats (112 citations) Inbred male Lewis rats (13 citations) Adult male Lewis rats (7 citations) Wild-type male Lewis rats (3 citations) Male Lewis and Brown Norway rats (2 citations)

85 protocols using male lewis rats

Fetal Motor Neuron Transplantation in Rats

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The animal rearing management was carried out according to the Kitasato University Faculty of Veterinary Medicine Animal Experiment Guideline and Manual for Rearing and Management of Experimental Animals (Approval No: 17-127, 18-127, 19-085). The rats were housed in cages under temperature and light-controlled conditions in a 12-hour cycle and were provided with fresh food and water ad libitum.
In Experiment 1, we used three pregnant female Lewis rats on gestation day 15 (E15) (Japan Charles River Laboratories, Kanagawa, Japan) to obtain fetal MNB. As recipient rats (organ recipient animals), we used 12 male Lewis rats (Japan Charles River Laboratories) aged 11 weeks, with a body weight of 309.0 ± 11.4 g.
In Experiment 2, we used three pregnant female Lewis rats on E15 (Japan Charles River Laboratories), and as recipient rats, 18 male Lewis rats (Japan Charles River Laboratories) aged 9 weeks, with a body weight of 243.0 ± 7.5 g.
In Experiment 3, we used three pregnant female Lewis rats on E15 (Japan Charles River Laboratories), and as recipient rats, 9 male Lewis rats (Japan Charles River Laboratories), aged 10 weeks, with a body weight of 292.8 ± 7.6 g.

Nishi K., Haji T., Matsumoto T., Hayakawa C., Maeda K., Okano S., Yokoo T., & Iwai S. (2020). Reconstruction of the urinary tract at the appropriate time reduces fibrosis of the metanephros in rats as judged by imaging.

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Rheumatoid Arthritis Rat Model Establishment and Analysis

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Male Lewis rats (n = 48, 6–8-week-old, 200 ± 20 g in weight) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (production license no. SCXK 2016-0006, Beijing, China). All animals were maintained under specific pathogen-free conditions in a temperature-controlled room at a constant temperature of 24 ± 1 °C with a 12 h light/dark cycle and had free access to the standard rodent diet and water ad libitum. The study was approved by the Research Ethics Committee of the Institute of Basic Theory of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, China (SYXK 2016-0021, Beijing, China). All animal studies were treated in accordance with the guidelines and regulations for the use and care of animals of the Center for Laboratory Animal Care, China Academy of Chinese Medical Sciences.
A total of 48 rats were randomly divided into three groups: ① normal control group (n = 16); ② AIA-Cold (RA-Cold model group, n = 16); ③ AIA-Hot (RA-Hot model group, n = 16). Among them, AIA-Cold and AIA-Hot rat models were established and evaluated according to our previous studies [9 (link), 11 (link)]. Urine and serum samples were collected 28th d after Freund complete adjuvant stimulation, after centrifugation at 3000 rpm for 15 min, the supernatant was reserved and stored at − 80 °C for subsequent metabonomics analysis.

Ding Z., Chen W., Wu H., Li W., Mao X., Su W., Zhang Y, & Lin N. (2023). Integrative network fusion-based multi-omics study for biomarker identification and patient classification of rheumatoid arthritis. Chinese Medicine, 18, 48.

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Experimental Autoimmune Myocarditis in Rats

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Male Lewis rats (180‐200 g), aged 6‐8 weeks, purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China) were maintained in Xiamen University animal experimental centre. All animal care and experiments were performed in accordance with procedures approved by the Animal Care and Use Committee of Xiamen University. Porcine cardiac myosin was prepared from the ventricular muscle of porcine hearts as previously described.29 To produce EAM, each rat was immunized on day 0 with 0.2 mL emulsion containing 1 mg porcine cardiac myosin with an equal volume of complete Freund's adjuvant supplemented with mycobacterium tuberculosis H37RA at a concentration of 10 mg/mL by a single subcutaneous injection in both footpads. The rats in the control group were only immunized with complete Freund's adjuvant.

Zhong C., Chang H., Wu Y., Zhou L., Wang Y., Wang M., Wu P., Qi Z, & Zou J. (2018). Up‐regulated Cx43 phosphorylation at Ser368 prolongs QRS duration in myocarditis. Journal of Cellular and Molecular Medicine, 22(7), 3537-3547.

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Xenogeneic Bone Marrow Mesenchymal Stem Cell Transplantation

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Inbred adult male Brown Norway (BN) rats (180–200 g) and male Lewis rats (200–220 g for SBTx and 100–120 g for BMMSCs) were obtained from Vital River Company (Beijing, China). Animals were housed individually in standard animal facilities, maintained on a 12-h light/dark cycle and provided with commercially available chow and tap water ad libitum for 3 days before testing. Food was withheld from both donor and recipient animals for 24 h prior to surgery. All experimental procedures were carried out in accordance with the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (NIH publication 86–23, revised 1985), and the protocols were approved by the Animal Care and Research Committee of Tianjin First Central Hospital, Tianjin, China (Permit Number: E20130605-001A). All surgery was performed under chloral hydrate anesthesia, and all efforts were made to minimize animal suffering.

Yang Y., Song H.L., Zhang W., Wu B.J., Fu N.N., Zheng W.P., Don C, & Shen Z.Y. (2014). Reduction of Acute Rejection by Bone Marrow Mesenchymal Stem Cells during Rat Small Bowel Transplantation. PLoS ONE, 9(12), e114528.

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Lewis Rat Ophthalmic Research Protocol

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Male Lewis rats (6–8 weeks old) purchased from Vital River (Beijing, China) were housed under pathogen-free conditions. All procedures involving rats were approved by the Laboratory Animal Care and Use Committee of Tianjin Medical University, and conformed to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.

Bai L., Shao H., Wang H., Zhang Z., Su C., Dong L., Yu B., Chen X., Li X, & Zhang X. (2017). Effects of Mesenchymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis. Scientific Reports, 7, 4323.

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Animal Model of Therapeutic Intervention

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Male Lewis rats, 6–8 weeks old (160–190 g), were purchased from the Vital River Corporation (Beijing, China). All animals were acclimated to the vivarium environment and were maintained under temperature-controlled conditions and a 12-h light/dark cycle for 1 week. Food and water were provided ad libitum. Animals were randomly assigned to three groups (preventative, therapeutic, or control, n = 6 per group) and each group was repeated 3 times. All efforts were made to minimize the number of animals used and any suffering they might experience. The experimental procedures were approved by the Animal Ethics Committee of the Tianjin Medical University.

Han R., Xiao J., Zhai H, & Hao J. (2016). Dimethyl fumarate attenuates experimental autoimmune neuritis through the nuclear factor erythroid-derived 2-related factor 2/hemoxygenase-1 pathway by altering the balance of M1/M2 macrophages. Journal of Neuroinflammation, 13, 97.

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Liver Transplantation in Rat Model

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Specific-pathogen-free healthy adult inbred male Brown-Norway (BN) rats (n=45) and male Lewis rats (n=35) were purchased from the Vital River Laboratories Animal Technology, Co., Ltd. (Beijing, China). The rats were housed individually in standard animal facilities at 18–26°C with a 12-h light/dark cycle and were provided with commercially available chow and tap water ad libitum. BM-MSCs were extracted from syngeneic inbred male BN rats (80–100 g; 4–5 weeks old). Inbred male Lewis rats (210–250 g; 8–10 weeks old) were the liver transplantation donors, and inbred male BN rats (210–250 g; 8–10 weeks old) were the recipients. The difference in body weight between the donor and the recipient of each pair did not exceed 10 g. Food was withheld from the recipient animals for 12 h before surgery, but was not withheld from the donor animals. The study protocol was approved by the Animal Care and Research Committee of Tianjin First Central Hospital (Tianjin, China). All surgeries and sacrifices were performed under 5% chloral hydrate anesthesia (0.5 ml/100 g). Every effort was made to minimize animal suffering.

Wang R., Shen Z., Yang L., Yin M., Zheng W., Wu B., Liu T, & Song H. (2017). Protective effects of heme oxygenase-1-transduced bone marrow-derived mesenchymal stem cells on reduced-size liver transplantation: Role of autophagy regulated by the ERK/mTOR signaling pathway. International Journal of Molecular Medicine, 40(5), 1537-1548.

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Experimental Autoimmune Myocarditis Induction

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Male Lewis rats (180–200g), aged 6–8 weeks, purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China) were maintained in Xiamen University animal experiment center. Cardiac myosin was prepared from the ventricular muscle of porcine hearts as previously described [62 (link)]. To produce EAM, each rat was immunized on day 0 with 0.2 mL emulsion containing 1 mg cardiac myosin with an equal volume of complete Freud's adjuvant supplemented with mycobacterium tuberculosis H37RA at a concentration of 10 mg/mL by a single subcutaneous injection in both footpads [62 (link)]. The rats in the control group were only immunized with complete Freund's adjuvant. All animal care and experiments were performed in accordance with procedures approved by the Animal Care and Use Committee of Xiamen University. The relative mRNA and protein levels were measured at week 3 of EAM.

Zhong C., Wu Y., Chang H., Liu C., Zhou L., Zou J, & Qi Z. (2017). Effect of PKC inhibitor on experimental autoimmune myocarditis in Lewis rats. Oncotarget, 8(33), 54187-54198.

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Establishing Rat Model for Research

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Male Lewis rats (n=24; aged 8 weeks; weight 180–200 g) were purchased from Vital River Laboratories Co., Ltd. (Beijing, China) and were maintained in our animal facilities with air-conditioning at Shantou University Medical College, under constant temperature and humidity conditions with a 12:12-h light-dark cycle. All rats had free access to food and water. Throughout the studies, all animals were treated in accordance with the institutional guidelines for animal experiments. Ethical approval was obtained from the Institutional Review Board of Shantou University Medical College (Shantou, China).

Zhang C., Zhou G., Cai C., Li J., Chen F., Xie L., Wang W., Zhang Y., Lai X, & Ma L. (2017). Human umbilical cord mesenchymal stem cells alleviate acute myocarditis by modulating endoplasmic reticulum stress and extracellular signal regulated 1/2-mediated apoptosis. Molecular Medicine Reports, 15(6), 3515-3520.

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Animal Housing and Acclimatization

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Male Lewis rats (200 ± 20 g, 5 weeks old) and male C57BL/6J mice (15 ± 2 g, 4 weeks old) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). Male DBA1/J mice (15 ± 2 g, 7 weeks old) were obtained from Shanghai SLAC Laboratory Animal Technology Co., Ltd. (Shanghai, China). All animals were housed in specific pathogen-free conditions at a temperature of 18–23 °C with 40–60% humidity under standard 12-h light/12-h dark cycle and allowed free access to food and water.

Han H., Xing J., Chen W., Jia J, & Li Q. (2023). Fluorinated polyamidoamine dendrimer-mediated miR-23b delivery for the treatment of experimental rheumatoid arthritis in rats. Nature Communications, 14, 944.

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